GeneTex
United States (US)

SLP76 antibody [SLP-76/03]

SLP76-antibody-SLP-7603__GTX14940-1_18121411_743.jpg

Cat No. GTX14940

Host Mouse
Clonality Monoclonal
Clone Name SLP-76/03
Isotype IgG2b
Application WB
Reactivity Human, Mouse, Pig
APPLICATION

Application Note

WB: Use at an assay dependent dilution. Predicted molecular weight: 76 kDa. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

60 kDa. ( Note )

Specificity/Sensitivity

The polyclonal antibody reacts with SLP76, a 76kDa cytosolic adaptor protein that is involved in signaling of various hematopoietic cells, such as T cells, mast cells or neutrophils.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline pH 7.4 containing 0.09% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Fusion protein: HEEPS RSRNHKTAKL PAPSIDRSTK PPLDRSLAPF DREPFTLGKK PPFSDKPSIP AGRSLGEHLP KIQKPPLPPT TERHERSSPL PGKKPPVPKH GWGPDRREND EDDVHQRPLP QPALLPMSSN TFPSRSTKPS PMNPLPSSHM PGAFSESNSS FPQSASLPPY FSQGPSNRPP IRAEGRNFPL PLPNKPRPPS PAEEENSLNE EWYVSYITRP EA

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Lymphocyte Cytosolic Protein 2,Slp-76,Slp76,Lcp2

Background

SLP76 (SH2 domain containing leukocyte protein of 76 kDa) is a hematopoietic cell-specific adaptor protein that is crucial for T-cell receptor (TCR) signaling, hemostasis and platelet function. TCR ligation and fibrinogen binding to integrin alpha 2 beta 3 stimulates the phosphorylation of the tyrosine residues in the amino terminus, and facilitates SLP76 binding to the SH2 domain of Vav, which can activate JNK. SLP76 also comprises a proline-rich domain region that associates with the SH3 domain of Grb2 linking SLP76 to the Ras to Raf to ERK1 & 2 signaling pathway, LAT, PLC gamma, Fyn-binding protein (SLAP130), the SH2 containing phosphatase 1 and Nck, which mediates the regulation of cytoskeletal actin polymerization. Phosphorylation of tyrosine 145 has been shown to be important for optimal SLP76 function.

Database

DATA IMAGES
SLP76-antibody-SLP-7603__GTX14940-1_18121411_743.jpg

GTX14940 WB Image

Western Blot analysis (reducing conditions) of whole cell lysate using anti-SLP76 (GTX14940).

Package List Price ($)
$ 319