GeneTex
United States (US)

SLP76 antibody

SLP76-antibody__GTX14942-1_18121411_483.jpg

Cat No. GTX14942

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

WB: Use at an assay dependent dilution. Predicted molecular weight: 64 kDa. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

60 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline pH 7.4 containing 15mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Fusion protein: HEEPS RSRNHKTAKL PAPSIDRSTK PPLDRSLAPF DREPFTLGKK PPFSDKPSIP AGRSLGEHLP KIQKPPLPPT TERHERSSPL PGKKPPVPKH GWGPDRREND EDDVHQRPLP QPALLPMSSN TFPSRSTKPS PMNPLPSSHM PGAFSESNSS FPQSASLPPY FSQGPSNRPP IRAEGRNFPL PLPNKPRPPS PAEEENSLNE EWYVSYITRP EA

Purification

>95% by SDS-PAGE
Purified from rabbit antisera by precipitation methods.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Lymphocyte Cytosolic Protein 2,Slp-76,Slp76,Lcp2

Background

SLP76 (SH2 domain containing leukocyte protein of 76 kDa) is a hematopoietic cell-specific adaptor protein that is crucial for T-cell receptor (TCR) signaling, hemostasis and platelet function. TCR ligation and fibrinogen binding to integrin alpha 2 beta 3 stimulates the phosphorylation of the tyrosine residues in the amino terminus, and facilitates SLP76 binding to the SH2 domain of Vav, which can activate JNK. SLP76 also comprises a proline-rich domain region that associates with the SH3 domain of Grb2 linking SLP76 to the Ras to Raf to ERK1 & 2 signaling pathway, LAT, PLC gamma, Fyn-binding protein (SLAP130), the SH2 containing phosphatase 1 and Nck, which mediates the regulation of cytoskeletal actin polymerization. Phosphorylation of tyrosine 145 has been shown to be important for optimal SLP76 function.

Database

DATA IMAGES
SLP76-antibody__GTX14942-1_18121411_483.jpg

GTX14942 IHC-P Image

Immunohistochemistry staining of human thymus (paraffin sections) with anti-SLP76 (GTX14942).

Package List Price ($)
$ 319