GeneTex
United States (US)

SLU7 antibody

GTX106861_39981_IHC_19011506_823.jpg
GTX106861_39981_IFA_19011506_187.jpg
GTX106861_39981_WB_B_19011506_487.jpg

Cat No. GTX106861

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

293T transfected SLU7 recombinant protein

Predict Reactivity

Mouse, Rat, Bovine, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human SLU7. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

9G8 antibody, MGC9280 antibody, hSlu7 antibody, SLU7 antibody, zinc knuckle motif containing antibody, splicing factor antibody, pre-mRNA-splicing factor SLU7 antibody, step II splicing factor SLU7 antibody, SLU7 splicing factor homolog (S. cerevisiae) antibody

Cellular Localization

Nucleus , Cytoplasm

Background

Pre-mRNA splicing occurs in two sequential transesterification steps. The protein encoded by this gene is a splicing factor that has been found to be essential during the second catalytic step in the pre-mRNA splicing process. It associates with the spliceosome and contains a zinc knuckle motif that is found in other splicing factors and is involved in protein-nucleic acid and protein-protein interactions. [provided by RefSeq]

Database

DATA IMAGES
GTX106861_39981_IHC_19011506_823.jpg

GTX106861 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma, using SLU7(GTX106861) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX106861_39981_IFA_19011506_187.jpg

GTX106861 ICC/IF Image

SLU7 antibody detects SLU7 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SLU7 protein stained by SLU7 antibody (GTX106861) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX106861_39981_WB_B_19011506_487.jpg

GTX106861 WB Image

Sample (30 ug of whole cell lysate)
A: Non-transfected 293T lysates
B: hSLU7 transfected 293T lysates
7.5% SDS PAGE
GTX106861 diluted at 1:10000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169