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SLU7 antibody

Anti-SLU7 antibody used in Western Blot (WB). GTX118180
Anti-SLU7 antibody used in IHC (Paraffin sections) (IHC-P). GTX118180
Anti-SLU7 antibody used in IHC (Paraffin sections) (IHC-P). GTX118180
Anti-SLU7 antibody used in Western Blot (WB). GTX118180

Cat No. GTX118180

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

A431 , HeLa , HepG2 , 293T transfected SLU7 recombinant protein

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SLU7. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11173224

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

SLU7 homolog, splicing factor , 9G8 , hSlu7

Cellular Localization

Nucleus , Cytoplasm

Background

Pre-mRNA splicing occurs in two sequential transesterification steps. The protein encoded by this gene is a splicing factor that has been found to be essential during the second catalytic step in the pre-mRNA splicing process. It associates with the spliceosome and contains a zinc knuckle motif that is found in other splicing factors and is involved in protein-nucleic acid and protein-protein interactions. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SLU7 antibody used in Western Blot (WB). GTX118180

GTX118180 WB Image

Sample (30 μg of whole cell lysate)
A: Non-transfected 293T lysates 
B: hSLU7 transfected 293T lysates
7.5% SDS PAGE
GTX118180 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SLU7 antibody used in IHC (Paraffin sections) (IHC-P). GTX118180

GTX118180 IHC-P Image

Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using SLU7(GTX118180) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SLU7 antibody used in IHC (Paraffin sections) (IHC-P). GTX118180

GTX118180 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using SLU7(GTX118180) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SLU7 antibody used in Western Blot (WB). GTX118180

GTX118180 WB Image

Sample (30 μg of whole cell lysate)
A: A431
B: HeLa
C: HepG2
7.5% SDS PAGE
GTX118180 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf