APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000-1:10000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
hSLU7 transfected 293T , A431 , HeLa , HepG2
Predict Reactivity
Rat, Zebrafish, Bovine, Chicken, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human SLU7. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_11173224
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
SLU7 homolog, splicing factor , 9G8 , hSlu7
Cellular Localization
Nucleus , Cytoplasm
Background
Pre-mRNA splicing occurs in two sequential transesterification steps. The protein encoded by this gene is a splicing factor that has been found to be essential during the second catalytic step in the pre-mRNA splicing process. It associates with the spliceosome and contains a zinc knuckle motif that is found in other splicing factors and is involved in protein-nucleic acid and protein-protein interactions. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX118180 IHC-P Image
Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using SLU7(GTX118180) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX118180 IHC-P Image
Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using SLU7(GTX118180) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX118180 WB Image
Sample (30 μg of whole cell lysate)
A: A431
B: HeLa
C: HepG2
7.5% SDS PAGE
GTX118180 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX118180 WB Image
Sample (30 μg of whole cell lysate)
A: Non-transfected 293T lysates
B: hSLU7 transfected 293T lysates
7.5% SDS PAGE
GTX118180 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for SLU7 antibody (GTX118180). Be the first to share your publications with this product.
REVIEW
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