GeneTex
United States (US)

SLU7 antibody

Anti-SLU7 antibody used in Western Blot (WB). GTX125935
Anti-SLU7 antibody used in IHC (Paraffin sections) (IHC-P). GTX125935

Cat No. GTX125935

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

293T transfected recombinant avian slu7 protein

Predict Reactivity

Chicken(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of avian avian Slu7. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Slu7 Homolog, Splicing Factor,Slu7

Cellular Localization

Nucleus

Background

Participates in the second catalytic step of pre-mRNA splicing, when the free hydroxyl group of exon I attacks the 3'-splice site to generate spliced mRNA and the excised lariat intron. Required for holding exon 1 properly in the spliceosome and for correct AG identification when more than one possible AG exists in 3'-splicing site region.

Database

DATA IMAGES
Anti-SLU7 antibody used in Western Blot (WB). GTX125935

GTX125935 WB Image

Sample (30 ug of whole cell lysate)
A: Non-transfected 293T lysates
B: Avian slu7 transfected 293T lysates
7.5% SDS PAGE
GTX125935 diluted at 1:5000

Anti-SLU7 antibody used in IHC (Paraffin sections) (IHC-P). GTX125935

GTX125935 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using avian Slu7 antibody(GTX125935) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169