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SLU7 antibody

Anti-SLU7 antibody used in Western Blot (WB). GTX125935
Anti-SLU7 antibody used in IHC (Paraffin sections) (IHC-P). GTX125935

Cat. No. GTX125935

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all SLU7 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

Avian slu7 protein transfected 293T

Predict Reactivity

Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Avian

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of avian Slu7. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11174342

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SLU7 homolog, splicing factor

Cellular Localization

Nucleus

Background

Participates in the second catalytic step of pre-mRNA splicing, when the free hydroxyl group of exon I attacks the 3'-splice site to generate spliced mRNA and the excised lariat intron. Required for holding exon 1 properly in the spliceosome and for correct AG identification when more than one possible AG exists in 3'-splicing site region.

Database

Research Area

DATA IMAGES

Anti-SLU7 antibody used in Western Blot (WB). GTX125935

GTX125935 WB Image

Sample (30 ug of whole cell lysate)
A: Non-transfected 293T lysates
B: Avian slu7 transfected 293T lysates
7.5% SDS PAGE
GTX125935 diluted at 1:5000

Anti-SLU7 antibody used in IHC (Paraffin sections) (IHC-P). GTX125935

GTX125935 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using avian Slu7 antibody(GTX125935) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169