GeneTex
  • Country / Location Selection

United States (US)

SLU7 antibody

Anti-SLU7 antibody used in Western Blot (WB). GTX125955
Anti-SLU7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125955
Anti-SLU7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125955
Anti-SLU7 antibody used in IHC (Paraffin sections) (IHC-P). GTX125955

Cat No. GTX125955

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Mouse, Rat, Bovine, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human SLU7. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

SLU7 homolog, splicing factor , 9G8 , hSlu7

Cellular Localization

Nucleus , Cytoplasm

Background

Pre-mRNA splicing occurs in two sequential transesterification steps. The protein encoded by this gene is a splicing factor that has been found to be essential during the second catalytic step in the pre-mRNA splicing process. It associates with the spliceosome and contains a zinc knuckle motif that is found in other splicing factors and is involved in protein-nucleic acid and protein-protein interactions. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SLU7 antibody used in Western Blot (WB). GTX125955

GTX125955 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431
C: HeLa
D: HepG2
7.5% SDS PAGE
GTX125955 diluted at 1:5000

Anti-SLU7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125955

GTX125955 ICC/IF Image

Slu7 antibody detects Slu7 protein at nucleus by immunofluorescent analysis.
Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Slu7 protein stained by Slu7 antibody (GTX125955) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-SLU7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125955

GTX125955 ICC/IF Image

Slu7 antibody detects Slu7 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Slu7 protein stained by Slu7 antibody (GTX125955) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-SLU7 antibody used in IHC (Paraffin sections) (IHC-P). GTX125955

GTX125955 IHC-P Image

Slu7 antibody detects SLU7 protein at nucleus on BT483 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded BT483 xenograft.
Slu7 antibody (GTX125955) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

There are currently no references for SLU7 antibody (GTX125955). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for SLU7 antibody (GTX125955). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf