GeneTex
United States (US)

SLU7 (phospho Ser513/Ser515) antibody

GTX125960_41150_20150324_IFA_18121410_129.jpg
GTX125960_41150_20150525_IFA_18121410_157.jpg

Cat No. GTX125960

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Predict Reactivity

Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.04 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide corresponding to residues around human Slu7 (phospho Ser513/Ser515). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Slu7 Homolog, Splicing Factor,9G8,Hslu7,Slu7

Cellular Localization

Nucleus , Cytoplasm

Background

Pre-mRNA splicing occurs in two sequential transesterification steps. The protein encoded by this gene is a splicing factor that has been found to be essential during the second catalytic step in the pre-mRNA splicing process. It associates with the spliceosome and contains a zinc knuckle motif that is found in other splicing factors and is involved in protein-nucleic acid and protein-protein interactions. [provided by RefSeq]

Database

DATA IMAGES
GTX125960_41150_20150324_IFA_18121410_129.jpg

GTX125960 ICC/IF Image

Slu7 (phospho Ser513/Ser515) antibody detects Slu7 (phospho Ser513/Ser515) protein at nucleus by immunofluorescent analysis.
Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Slu7 (phospho Ser513/Ser515) protein stained by Slu7 (phospho Ser513/Ser515) antibody (GTX125960) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX125960_41150_20150525_IFA_18121410_157.jpg

GTX125960 ICC/IF Image

Slu7 (phospho Ser513/Ser515) antibody detects Slu7 (phospho Ser513/Ser515) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Slu7 (phospho Ser513/Ser515) protein stained by Slu7 (phospho Ser513/Ser515) antibody (GTX125960) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169