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SLUG antibody

Anti-SLUG antibody used in Western Blot (WB). GTX121924
Anti-SLUG antibody used in IHC (Paraffin sections) (IHC-P). GTX121924

Cat No. GTX121924

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, IHC

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

Positive Control

human SNAI2-transfected 293T cells

Predict Reactivity

Rat, Zebrafish, Sheep, Bovine, Dog, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SLUG. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10728929

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

snail family transcriptional repressor 2 , SLUG , SLUGH , SLUGH1 , SNAIL2 , WS2D

Cellular Localization

Nucleus

Background

This gene encodes a member of the Snail family of C2H2-type zinc finger transcription factors. The encoded protein acts as a transcriptional repressor that binds to E-box motifs and is also likely to repress E-cadherin transcription in breast carcinoma. This protein is involved in epithelial-mesenchymal transitions and has antiapoptotic activity. Mutations in this gene may be associated with sporatic cases of neural tube defects. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SLUG antibody used in Western Blot (WB). GTX121924

GTX121924 WB Image

SLUG antibody detects SNAI2 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of human SNAI2-transfected 293T cells
12% SDS-PAGE
SLUG antibody (GTX121924) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SLUG antibody used in IHC (Paraffin sections) (IHC-P). GTX121924

GTX121924 IHC-P Image

SLUG antibody detects SLUG protein at nucleus on mouse middle brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse middle brain.
SLUG antibody (GTX121924) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

REVIEW

SLUG antibody Cat No. GTX121924

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017.PMID: 28134285
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169