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SLUG antibody

Anti-SLUG antibody used in Western Blot (WB). GTX121924
Anti-SLUG antibody used in IHC (Paraffin sections) (IHC-P). GTX121924

Cat No. GTX121924

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IHC
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

Positive Control

human SNAI2-transfected 293T cells

Predict Reactivity

Rat, Zebrafish, Sheep, Bovine, Dog, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SLUG. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Snail Family Transcriptional Repressor 2,Slug,Slugh,Slugh1,Snail2,Ws2D,Snai2

Cellular Localization

Nucleus

Background

This gene encodes a member of the Snail family of C2H2-type zinc finger transcription factors. The encoded protein acts as a transcriptional repressor that binds to E-box motifs and is also likely to repress E-cadherin transcription in breast carcinoma. This protein is involved in epithelial-mesenchymal transitions and has antiapoptotic activity. Mutations in this gene may be associated with sporatic cases of neural tube defects. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-SLUG antibody used in Western Blot (WB). GTX121924

GTX121924 WB Image

SLUG antibody detects SNAI2 protein by western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg whole cell lysate/extract of human SNAI2-transfected 293T cells
12% SDS-PAGE
SLUG antibody (GTX121924) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SLUG antibody used in IHC (Paraffin sections) (IHC-P). GTX121924

GTX121924 IHC-P Image

SLUG antibody detects SLUG protein at nucleus on mouse middle brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse middle brain.
SLUG antibody (GTX121924) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169