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SMAD2 / SMAD3 antibody

Orthogonal Overexpression
Anti-SMAD2 / SMAD3 antibody used in ChIP assay (ChIP assay). GTX111123
Anti-SMAD2 / SMAD3 antibody used in Western Blot (WB). GTX111123
Anti-SMAD2 / SMAD3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111123
Anti-SMAD2 / SMAD3 antibody used in Western Blot (WB). GTX111123
Anti-SMAD2 / SMAD3 antibody used in IHC (Paraffin sections) (IHC-P). GTX111123
Anti-SMAD2 / SMAD3 antibody used in Immunoprecipitation (IP). GTX111123
Anti-SMAD2 / SMAD3 antibody used in Western Blot (WB). GTX111123
Anti-SMAD2 / SMAD3 antibody used in Western Blot (WB). GTX111123
Anti-SMAD2 / SMAD3 antibody used in Western Blot (WB). GTX111123
Anti-SMAD2 / SMAD3 antibody used in Western Blot (WB). GTX111123
Anti-SMAD2 / SMAD3 antibody used in Western Blot (WB). GTX111123

Cat. No. GTX111123

Reference 10
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS, IP, ChIP assay

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all SMAD2 / SMAD3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
FACS Assay dependent
IP 1:1000-1:1000
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

mouse brain , rat brain , SMAD2 and SMAD3-transfected 293T

Predict Reactivity

Bovine, Dog, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.75 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SMAD2 / SMAD3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2038001

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DKFZp586N0721 antibody , DKFZp686J10186 antibody , HSPC193 antibody , HsT17436 antibody , JV15-2 antibody , MADH3 antibody , MGC60396 antibody , SMAD3 antibody , hSMAD3 antibody , mad homolog JV15-2 antibody , MAD homolog 3 antibody , hMAD-3 antibody , MAD , mothers against decapentaplegic homolog 3 antibody , SMA- and MAD-related protein 3 antibody , mad protein homolog antibody , mothers against DPP homolog 3 antibody , mothers against decapentaplegic homolog 3 antibody , mad3 antibody , SMAD , mothers against DPP homolog 3 antibody , SMAD family member 3 antibody

Cellular Localization

Cytoplasm , Nucleus

Research Area

DATA IMAGES

Anti-SMAD2 / SMAD3 antibody used in ChIP assay (ChIP assay). GTX111123

GTX111123 ChIP assay Image

SMAD2 / SMAD3 antibody immunoprecipitates SMAD2 / SMAD3 protein-DNA in ChIP experiments. ChIP Sample: HeLa whole cell lysate/extract A. 5 μg preimmune rabbit IgG B. 5 μg of SMAD2 / SMAD3 antibody (GTX111123) The precipitated DNA was detected by PCR with primer set targeting to smad7 promoter.

Anti-SMAD2 / SMAD3 antibody used in Western Blot (WB). GTX111123

GTX111123 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SMAD2 / SMAD3 antibody (GTX111123) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SMAD2 / SMAD3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111123

GTX111123 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using SMAD2 / SMAD3 (GTX111123) antibody at 1:200 dilution.

Anti-SMAD2 / SMAD3 antibody used in Western Blot (WB). GTX111123

GTX111123 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SMAD2 / SMAD3 antibody (GTX111123) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SMAD2 / SMAD3 antibody used in IHC (Paraffin sections) (IHC-P). GTX111123

GTX111123 IHC-P Image

SMAD2 / SMAD3 antibody detects SMAD2 / SMAD3 protein at cytosol and nucleus on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
SMAD2 / SMAD3 antibody (GTX111123) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SMAD2 / SMAD3 antibody used in Immunoprecipitation (IP). GTX111123

GTX111123 IP Image

Immunoprecipitation of SMAD2 / SMAD3 protein from HeLa whole cell extracts using 2 μg of SMAD2 / SMAD3 antibody (GTX111123).
Western blot analysis was performed using SMAD2 / SMAD3 antibody (GTX111123) diluted at 1:1000.
EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01) was used to detect the primary antibody.

Anti-SMAD2 / SMAD3 antibody used in Western Blot (WB). GTX111123

GTX111123 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SMAD2 / SMAD3 antibody (GTX111123) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SMAD2 / SMAD3 antibody used in Western Blot (WB). GTX111123

GTX111123 WB Image

Orthogonal

The data was published in the 2022 in Front Pharmacol. PMID: 35517780

Anti-SMAD2 / SMAD3 antibody used in Western Blot (WB). GTX111123

GTX111123 WB Image

The data was published in the 2022 in Front Pharmacol. PMID: 35517780

Anti-SMAD2 / SMAD3 antibody used in Western Blot (WB). GTX111123

GTX111123 WB Image

The data was published in the 2022 in Front Pharmacol. PMID: 35517780

Anti-SMAD2 / SMAD3 antibody used in Western Blot (WB). GTX111123

GTX111123 WB Image

The data was published in the 2022 in Front Pharmacol. PMID: 35517780

REFERENCE

Application Reference

Zheng H et al. J Orthop Translat 2024; 44 : 35-46 TCF12 regulates the TGF-β/Smad2/3 signaling pathway to accelerate the progression of osteoarthritis by targeting CXCR4.
J Orthop Translat 2024; 44 : 35-46 TCF12 regulates the TGF-β/Smad2/3 signaling pathway to accelerate the progression of osteoarthritis by targeting CXCR4
Huang-Chi Chen et al. Front Pharmacol 2022; 13 : 877125 Effects of Montelukast on Arsenic-Induced Epithelial-Mesenchymal Transition and the Role of Reactive Oxygen Species Production in Human Bronchial Epithelial Cells.
Application : WB
Reactivity : Human
Huang YT et al. Cell Death Dis 2021; 12 (10) : 880 USP7 facilitates SMAD3 autoregulation to repress cancer progression in p53-deficient lung cancer.
Application : WB
Reactivity : Human
Traxel S et al. Cancer Immunol Immunother 2021; Support of BCP-ALL-cells by autologous bone marrow Th-cells involves induction of AID expression but not widespread AID off-target mutagenesis.
Application : WB
Reactivity : Human
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REVIEW

SMAD2 / SMAD3 antibody Cat. No. GTX111123

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 2 users reviews)
Anti-SMAD2 / SMAD3 antibody used in Western Blot (WB). GTX111123
Date : Anonymous submitted on 20-Dec-2022
Score :
Application Tested : WB
Sample Species : Ms
Sample : NIH3T3
Amount used : 30ug
Blocking : 25°C, 1Hr
Primary Antibody : Undiluted, 4°C, 16Hr
Anti-SMAD2 / SMAD3 antibody used in Western Blot (WB). GTX111123
Date : Anonymous submitted on 24-May-2022
Score :
Application Tested : WB
Sample Species : Hu
Sample : human prostate cancer cell lines, PC3 & C4-2B
Primary Antibody : 1:6000, °C, Hr
Submit a Review
Application Reference
Zheng H et al. J Orthop Translat 2024; 44:35-46 TCF12 regulates the TGF-β/Smad2/3 signaling pathway to accelerate the progression of osteoarthritis by targeting CXCR4.
J Orthop Translat 2024; 44:35-46 TCF12 regulates the TGF-β/Smad2/3 signaling pathway to accelerate the progression of osteoarthritis by targeting CXCR4
Huang-Chi Chen et al. Front Pharmacol 2022; 13:877125 Effects of Montelukast on Arsenic-Induced Epithelial-Mesenchymal Transition and the Role of Reactive Oxygen Species Production in Human Bronchial Epithelial Cells.
Application : WB
Reactivity : Human
Huang YT et al. Cell Death Dis 2021; 12 (10):880 USP7 facilitates SMAD3 autoregulation to repress cancer progression in p53-deficient lung cancer.
Application : WB
Reactivity : Human
Traxel S et al. Cancer Immunol Immunother 2021; Support of BCP-ALL-cells by autologous bone marrow Th-cells involves induction of AID expression but not widespread AID off-target mutagenesis.
Application : WB
Reactivity : Human
Chen HY et al. Antioxidants (Basel) 2021; 10 (1) Characterization of TGF-β by Induced Oxidative Stress in Human Trabecular Meshwork Cells.
Application : WB
Reactivity : Human
Shih TW et al. Sci Rep 2020; 10 (1):10857 An important role of PHRF1 in dendritic architecture and memory formation by modulating TGF-β signaling.
Application : WB
Reactivity : Mouse
Katoh D et al. Am J Pathol 2020; 190 (10):2123-2135 Tenascin-C Induces Phenotypic Changes in Fibroblasts to Myofibroblasts with High Contractility through the Integrin αvβ1/Transforming Growth Factor β/SMAD Signaling Axis in Human Breast Cancer.
Application : ICC/IF
Reactivity : Human
Ye J et al. Front Oncol 2019; 9:1251 miR-4666-3p and miR-329 Synergistically Suppress the Stemness of Colorectal Cancer Cells via Targeting TGF-帣/Smad Pathway.
Application : FACS
Reactivity : Human
Thiyagarajan V et al. PLoS One 2015; 10 (10):e0141285 Antroquinonol Targets FAK-Signaling Pathway Suppressed Cell Migration, Invasion, and Tumor Growth of C6 Glioma.
Application : WB
Reactivity : Rat
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169