GeneTex
  • Country / Location Selection

United States (US)

SMAD2 / SMAD3 antibody

Anti-SMAD2 / SMAD3 antibody used in ChIP assay (ChIP assay). GTX111123
Anti-SMAD2 / SMAD3 antibody used in Western Blot (WB). GTX111123
Anti-SMAD2 / SMAD3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111123
Anti-SMAD2 / SMAD3 antibody used in Western Blot (WB). GTX111123
Anti-SMAD2 / SMAD3 antibody used in Immunoprecipitation (IP). GTX111123
Anti-SMAD2 / SMAD3 antibody used in IHC (Paraffin sections) (IHC-P). GTX111123
Anti-SMAD2 / SMAD3 antibody used in Western Blot (WB). GTX111123

Cat No. GTX111123

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, FACS, IP, ChIP assay

Reactivity

Human, Mouse, Rat
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
FACS Assay dependent
IP 1:1000-1:1000
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , mouse brain , rat brain , SMAD2 and SMAD3-transfected 293T

Predict Reactivity

Bovine, Dog, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.75 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SMAD2 / SMAD3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2038001

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

DKFZp586N0721 antibody , DKFZp686J10186 antibody , HSPC193 antibody , HsT17436 antibody , JV15-2 antibody , MADH3 antibody , MGC60396 antibody , SMAD3 antibody , hSMAD3 antibody , mad homolog JV15-2 antibody , MAD homolog 3 antibody , hMAD-3 antibody , MAD , mothers against decapentaplegic homolog 3 antibody , SMA- and MAD-related protein 3 antibody , mad protein homolog antibody , mothers against DPP homolog 3 antibody , mothers against decapentaplegic homolog 3 antibody , mad3 antibody , SMAD , mothers against DPP homolog 3 antibody , SMAD family member 3 antibody

Cellular Localization

Cytoplasm , Nucleus

Research Area

DATA IMAGES

Anti-SMAD2 / SMAD3 antibody used in ChIP assay (ChIP assay). GTX111123

GTX111123 ChIP assay Image

SMAD2 / SMAD3 antibody immunoprecipitates SMAD2 / SMAD3 protein-DNA in ChIP experiments. ChIP Sample: HeLa whole cell lysate/extract A. 5 μg preimmune rabbit IgG B. 5 μg of SMAD2 / SMAD3 antibody (GTX111123) The precipitated DNA was detected by PCR with primer set targeting to smad7 promoter.

Anti-SMAD2 / SMAD3 antibody used in Western Blot (WB). GTX111123

GTX111123 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SMAD2 / SMAD3 antibody (GTX111123) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SMAD2 / SMAD3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111123

GTX111123 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using SMAD2 / SMAD3 (GTX111123) antibody at 1:200 dilution.

Anti-SMAD2 / SMAD3 antibody used in Western Blot (WB). GTX111123

GTX111123 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SMAD2 / SMAD3 antibody (GTX111123) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SMAD2 / SMAD3 antibody used in Immunoprecipitation (IP). GTX111123

GTX111123 IP Image

Immunoprecipitation of SMAD2 / SMAD3 protein from HeLa whole cell extracts using 2 μg of SMAD2 / SMAD3 antibody (GTX111123).
Western blot analysis was performed using SMAD2 / SMAD3 antibody (GTX111123) diluted at 1:1000.
EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01) was used to detect the primary antibody.

Anti-SMAD2 / SMAD3 antibody used in IHC (Paraffin sections) (IHC-P). GTX111123

GTX111123 IHC-P Image

SMAD2 / SMAD3 antibody detects SMAD2 / SMAD3 protein at cytosol and nucleus on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
SMAD2 / SMAD3 antibody (GTX111123) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SMAD2 / SMAD3 antibody used in Western Blot (WB). GTX111123

GTX111123 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SMAD2 / SMAD3 antibody (GTX111123) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

SMAD2 / SMAD3 antibody Cat No. GTX111123

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 24-May-2022
Score :
Application Tested : WB
Sample Species : Hu
Sample : human prostate cancer cell lines, PC3 & C4-2B
Primary Antibody : 1:6000, °C, Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 379
$ 169