APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000-1:20000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| Assay dependent |
| 1:1000-1:1000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
mouse brain , rat brain , SMAD2 and SMAD3-transfected 293T
Predict Reactivity
Bovine, Dog, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.75 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human SMAD2 / SMAD3. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2038001
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
DKFZp586N0721 antibody , DKFZp686J10186 antibody , HSPC193 antibody , HsT17436 antibody , JV15-2 antibody , MADH3 antibody , MGC60396 antibody , SMAD3 antibody , hSMAD3 antibody , mad homolog JV15-2 antibody , MAD homolog 3 antibody , hMAD-3 antibody , MAD , mothers against decapentaplegic homolog 3 antibody , SMA- and MAD-related protein 3 antibody , mad protein homolog antibody , mothers against DPP homolog 3 antibody , mothers against decapentaplegic homolog 3 antibody , mad3 antibody , SMAD , mothers against DPP homolog 3 antibody , SMAD family member 3 antibody
Cellular Localization
Cytoplasm , Nucleus
Research Area
DATA IMAGES
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GTX111123 ChIP assay Image
SMAD2 / SMAD3 antibody immunoprecipitates SMAD2 / SMAD3 protein-DNA in ChIP experiments. ChIP Sample: HeLa whole cell lysate/extract A. 5 μg preimmune rabbit IgG B. 5 μg of SMAD2 / SMAD3 antibody (GTX111123) The precipitated DNA was detected by PCR with primer set targeting to smad7 promoter.
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GTX111123 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SMAD2 / SMAD3 antibody (GTX111123) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX111123 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using SMAD2 / SMAD3 (GTX111123) antibody at 1:200 dilution.
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GTX111123 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SMAD2 / SMAD3 antibody (GTX111123) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX111123 IHC-P Image
SMAD2 / SMAD3 antibody detects SMAD2 / SMAD3 protein at cytosol and nucleus on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
SMAD2 / SMAD3 antibody (GTX111123) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX111123 IP Image
Immunoprecipitation of SMAD2 / SMAD3 protein from HeLa whole cell extracts using 2 μg of SMAD2 / SMAD3 antibody (GTX111123).
Western blot analysis was performed using SMAD2 / SMAD3 antibody (GTX111123) diluted at 1:1000.
EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01) was used to detect the primary antibody.
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GTX111123 WB Image
Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SMAD2 / SMAD3 antibody (GTX111123) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX111123 WB Image
The data was published in the 2022 in Front Pharmacol. PMID: 35517780
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GTX111123 WB Image
The data was published in the 2022 in Front Pharmacol. PMID: 35517780
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GTX111123 WB Image
The data was published in the 2022 in Front Pharmacol. PMID: 35517780
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GTX111123 WB Image
The data was published in the 2022 in Front Pharmacol. PMID: 35517780
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REFERENCE
REVIEW
SMAD2 / SMAD3 antibody
Cat. No. GTX111123
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Rating
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( Average 5 based on 2 users reviews)
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Application
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Western Blot(WB)
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( Average 5 based on 2 users reviews)
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Date :
Anonymous submitted on 20-Dec-2022
Application Tested :
WB
Sample Species :
Ms
Sample :
NIH3T3
Amount used :
30ug
Blocking :
25°C, 1Hr
Primary Antibody :
Undiluted, 4°C, 16Hr
Date :
Anonymous submitted on 24-May-2022
Application Tested :
WB
Sample Species :
Hu
Sample :
human prostate cancer cell lines, PC3 & C4-2B
Primary Antibody :
1:6000, °C, Hr
