SMAD2 / SMAD3 (phospho Thr8) antibody [HL4327]

Specificity test was performed by coating the plate with recombinant SMAD2 non-phospho peptide, SMAD2 (phospho Thr8) peptide, SMAD3 non-phospho peptide, and SMAD3 (phospho Thr8) peptide (62.5 ng/mL). Coated protein was probed with SMAD2 / SMAD3 (phospho Thr8) antibody [HL4327] (GTX643574) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with SMAD2 / SMAD3 (phospho Thr8) antibody [HL4327] (GTX643574) diluted at 1:1000 and SMAD2 / SMAD3 antibody (GTX111123) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) C2C12 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with SMAD2 / SMAD3 (phospho Thr8) antibody [HL4327] (GTX643574) diluted at 1:1000 and SMAD2 / SMAD3 antibody (GTX111123) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.