SMAD2 antibody [2C10]

Cat. No. GTX83612

Host	Mouse
Clonality	Monoclonal
Clone Name	2C10
Isotype	IgG2b
Application	WB, ICC/IF, FACS
Reactivity	Human
Package	100 μl ($519)

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Datasheet
Document

View product citations for antibody GTX83612 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:2000
ICC/IF	1:100
FACS	1:100

Not tested in other applications.

Calculated MW

52 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.95 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human SMAD2 (NP_005892) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SMAD family member 2 , JV18 , JV18-1 , MADH2 , MADR2 , hMAD-2 , hSMAD2

Cellular Localization

Cytoplasm,Nucleus

Background

Transcriptional modulator activated by TGF-beta and activin type 1 receptor kinase. SMAD2 is a receptor-regulated SMAD (R-SMAD). May act as a tumor suppressor in colorectal carcinoma.

Database

Gene ID: 4087 SMAD2
UniProt: Q15796 SMAD2

Research Area

DATA IMAGES

GTX83612 WB Image

WB analysis of HEK293T cells transfected with SMAD2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83612 SMAD2 antibody [2C10].
Loading : 5 ug per lane

GTX83612 FACS Image

FACS analysis of Jurkat cells using GTX83612 SMAD2 antibody [2C10].
Red : Primary antibody
Blue : Negative control antibody

GTX83612 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with SMAD2 plasmid using GTX83612 SMAD2 antibody [2C10].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 519

Qty