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SMAD2 antibody [4G7]

Anti-SMAD2 antibody [4G7] used in Western Blot (WB). GTX83613
Anti-SMAD2 antibody [4G7] used in Flow cytometry (FACS). GTX83613
Anti-SMAD2 antibody [4G7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83613
Anti-SMAD2 antibody [4G7] used in Western Blot (WB). GTX83613

Cat No. GTX83613

Host

Mouse

Clonality

Monoclonal

Clone Name

4G7

Isotype

IgG2b

Application

WB, ICC/IF, FACS

Reactivity

Human
Package
100 μl ($419)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ICC/IF 1:100
FACS 1:100
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.92 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human SMAD2 (NP_005892) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

SMAD family member 2 , JV18 , JV18-1 , MADH2 , MADR2 , hMAD-2 , hSMAD2

Cellular Localization

Cytoplasm,Nucleus

Background

Transcriptional modulator activated by TGF-beta and activin type 1 receptor kinase. SMAD2 is a receptor-regulated SMAD (R-SMAD). May act as a tumor suppressor in colorectal carcinoma.

Database

Research Area

DATA IMAGES

Anti-SMAD2 antibody [4G7] used in Western Blot (WB). GTX83613

GTX83613 WB Image

WB analysis of HEK293T cells transfected with SMAD2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83613 SMAD2 antibody [4G7].
Loading : 5 ug per lane

Anti-SMAD2 antibody [4G7] used in Flow cytometry (FACS). GTX83613

GTX83613 FACS Image

FACS analysis of HEK293T cells transfected with either SMAD2 plasmid(Red) or empty vector control plasmid(Blue) using GTX83613 SMAD2 antibody [4G7].

Anti-SMAD2 antibody [4G7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83613

GTX83613 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with SMAD2 plasmid using GTX83613 SMAD2 antibody [4G7].

Anti-SMAD2 antibody [4G7] used in Western Blot (WB). GTX83613

GTX83613 WB Image

WB analysis of various cell lines using GTX83613 SMAD2 antibody [4G7].
Loading : 35 ug per lane
Dilution : 1:200

REFERENCE

There are currently no references for SMAD2 antibody [4G7] (GTX83613). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for SMAD2 antibody [4G7] (GTX83613). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf