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SMAD2 antibody [5G7]

Anti-SMAD2 antibody [5G7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60473
Anti-SMAD2 antibody [5G7] used in Western Blot (WB). GTX60473
Anti-SMAD2 antibody [5G7] used in ELISA (ELISA). GTX60473
Anti-SMAD2 antibody [5G7] used in Western Blot (WB). GTX60473
Anti-SMAD2 antibody [5G7] used in IHC (Paraffin sections) (IHC-P). GTX60473
Anti-SMAD2 antibody [5G7] used in Flow cytometry (FACS). GTX60473
Anti-SMAD2 antibody [5G7] used in IHC (Paraffin sections) (IHC-P). GTX60473

Cat No. GTX60473

Host

Mouse

Clonality

Monoclonal

Clone Name

5G7

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

Human, Mouse
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites, 0.03% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human SMAD2 expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

SMAD family member 2 , JV18 , JV18-1 , MADH2 , MADR2 , hMAD-2 , hSMAD2

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, May 2012]

Database

Research Area

DATA IMAGES

Anti-SMAD2 antibody [5G7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60473

GTX60473 ICC/IF Image

ICC/IF analysis of U251 cells using GTX60473 SMAD2 antibody [5G7].
Green : SMAD2
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

Anti-SMAD2 antibody [5G7] used in Western Blot (WB). GTX60473

GTX60473 WB Image

WB analysis of HEK293 (1) and SMAD2(AA: 20-254)-hIgGFc transfected HEK293 (2) cell lysate using GTX60473 SMAD2 antibody [5G7].

Anti-SMAD2 antibody [5G7] used in ELISA (ELISA). GTX60473

GTX60473 ELISA Image

ELISA analysis of antigen using GTX60473 SMAD2 antibody [5G7].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng

Anti-SMAD2 antibody [5G7] used in Western Blot (WB). GTX60473

GTX60473 WB Image

WB analysis of human SMAD2 (AA: 20-254) recombinant protein using GTX60473 SMAD2 antibody [5G7].

Anti-SMAD2 antibody [5G7] used in IHC (Paraffin sections) (IHC-P). GTX60473

GTX60473 IHC-P Image

IHC-P analysis of human cerebellum tissue using GTX60473 SMAD2 antibody [5G7].

Anti-SMAD2 antibody [5G7] used in Flow cytometry (FACS). GTX60473

GTX60473 FACS Image

FACS analysis of NIH3T3 cells using GTX60473 SMAD2 antibody [5G7].
Blue : SMAD2
Red : negative control

Anti-SMAD2 antibody [5G7] used in IHC (Paraffin sections) (IHC-P). GTX60473

GTX60473 IHC-P Image

IHC-P analysis of human liver cancer tissue using GTX60473 SMAD2 antibody [5G7].

REFERENCE

There are currently no references for SMAD2 antibody [5G7] (GTX60473). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for SMAD2 antibody [5G7] (GTX60473). Be the first to share your experience with this product.
SDS
Sodium Azide.pdf