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SMAD2 antibody [SP06-05]

Recombinant antibody
Anti-SMAD2 antibody [SP06-05] used in Western Blot (WB). GTX01187
Anti-SMAD2 antibody [SP06-05] used in Western Blot (WB). GTX01187
Anti-SMAD2 antibody [SP06-05] used in Western Blot (WB). GTX01187
Anti-SMAD2 antibody [SP06-05] used in Flow cytometry (FACS). GTX01187

Cat No. GTX01187

Host

Rabbit

Clonality

Monoclonal

Clone Name

SP06-05

Isotype

IgG

Application

WB, FACS

Reactivity

Human, Mouse, Rat
Package
100 μl ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:2000
FACS 1:50-1:100
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Observed MW

60 kDa. The observed M.W. is based on the following publication. PMID: 29963124

Product Note

This antibody is specific for SMAD2 protein, and it does not cross react with human SMAD1, SMAD3, SMAD4, SMAD5, or SMAD7 protein.

PROPERTIES

Form

Liquid

Buffer

1x TBS (pH7.4), 1% BSA, 40% Glycerol, 0.05% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

SMAD family member 2 , JV18 , JV181 , MADH2 , MADR2 , SMAD2 , hMAD2 , hSMAD2 , Smad2

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, May 2012]

Database

Research Area

DATA IMAGES

Anti-SMAD2 antibody [SP06-05] used in Western Blot (WB). GTX01187

GTX01187 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SMAD2 antibody [SP06-05] (GTX01187) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SMAD2 antibody [SP06-05] used in Western Blot (WB). GTX01187

GTX01187 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SMAD2 antibody [SP06-05] (GTX01187) diluted at 1:500.The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SMAD2 antibody [SP06-05] used in Western Blot (WB). GTX01187

GTX01187 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SMAD2 antibody [SP06-05] (GTX01187) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SMAD2 antibody [SP06-05] used in Flow cytometry (FACS). GTX01187

GTX01187 FACS Image

FACS analysis of NIH-3T3 cells using GTX01187 SMAD2 antibody [SP06-05].
Red : Primary antibody
Black : unlabelled control
Dilution : 1:50

REFERENCE

There are currently no references for SMAD2 antibody [SP06-05] (GTX01187). Be the first to share your publications with this product.

REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf