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SMAD2 antibody

Anti-SMAD2 antibody used in Western Blot (WB). GTX111075
Anti-SMAD2 antibody used in IHC (Paraffin sections) (IHC-P). GTX111075
Anti-SMAD2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111075
Anti-SMAD2 antibody used in IHC (Paraffin sections) (IHC-P). GTX111075
Anti-SMAD2 antibody used in Western Blot (WB). GTX111075

Cat No. GTX111075

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

293T , A431 , Jurkat , Raji , SMAD2-transfected 293T

Product Note

This antibody may recognize other Smad family members.

Predict Reactivity

Zebrafish, Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.55 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SMAD2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10730237

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

SMAD family member 2 , JV18 , JV18-1 , MADH2 , MADR2 , hMAD-2 , hSMAD2

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SMAD2 antibody used in Western Blot (WB). GTX111075

GTX111075 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SMAD2 antibody (GTX111075) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SMAD2 antibody used in IHC (Paraffin sections) (IHC-P). GTX111075

GTX111075 IHC-P Image

SMAD2 antibody detects SMAD2 protein at cytoplasm in mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
SMAD2 antibody (GTX111075) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SMAD2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111075

GTX111075 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using SMAD2(GTX111075) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-SMAD2 antibody used in IHC (Paraffin sections) (IHC-P). GTX111075

GTX111075 IHC-P Image

SMAD2 antibody detects SMAD2 protein at cytoplasm in rat testis by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
SMAD2 antibody (GTX111075) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SMAD2 antibody used in Western Blot (WB). GTX111075

GTX111075 WB Image

Sample (30 ug of whole cell lysate)
A: Jurkat
10% SDS PAGE
GTX111075 diluted at 1:500

REFERENCE

REVIEW

SMAD2 antibody Cat No. GTX111075

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS Genet in 2019.PMID: 31306413
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169