GeneTex
  • Country / Location Selection

United States (US)

SMAD2 antibody

Anti-SMAD2 antibody used in Immunoprecipitation (IP). GTX111131
Anti-SMAD2 antibody used in ChIP assay (ChIP assay). GTX111131
Anti-SMAD2 antibody used in Western Blot (WB). GTX111131
Anti-SMAD2 antibody used in Western Blot (WB). GTX111131
Anti-SMAD2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111131

Cat No. GTX111131

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IP, ChIP assay
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP 1:500-1:1000
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

HeLa , SMAD2-transfected 293T

Specificity/Sensitivity

This antibody may recognize other Smad family members.

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SMAD2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

SMAD family member 2,JV18,JV18-1,MADH2,MADR2,hMAD-2,hSMAD2

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-SMAD2 antibody used in Immunoprecipitation (IP). GTX111131

GTX111131 IP Image

SMAD2 antibody immunoprecipitates SMAD2 protein in IP experiments. IP Sample: 1000 µg HeLa whole cell lysate/extract A. 50 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of SMAD2protein by 2 μg of SMAD2antibody (GTX111131) 10% SDS-PAGE The immunoprecipitated SMAD2protein was detected by SMAD2antibody (GTX111131) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-SMAD2 antibody used in ChIP assay (ChIP assay). GTX111131

GTX111131 ChIP assay Image

SMAD2 antibody immunoprecipitates SMAD2 protein-DNA in ChIP experiments. ChIP Sample: HeLa whole cell lysate/extract A. 5 μg preimmune rabbit IgG B. 5 μg of SMAD2 antibody (GTX111131) The precipitated DNA was detected by PCR with primer set targeting to E-cadherin promoter.

Anti-SMAD2 antibody used in Western Blot (WB). GTX111131

GTX111131 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with SMAD2 antibody (GTX111131) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SMAD2 antibody used in Western Blot (WB). GTX111131

GTX111131 WB Image

Sample (30 µg of whole cell lysate)
A: HeLa
10% SDS PAGE
GTX111131 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SMAD2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111131

GTX111131 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using SMAD2(GTX111131) antibody at 1:200 dilution.

REFERENCE
REVIEW
There are currently no reviews for SMAD2 antibody (GTX111131). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169