Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SMAD2 (phospho Ser250) antibody [GT1291] (GTX03203) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

WB analysis of 293T cells were treated by PMA/TPA (200 nM) at 37ºC for 30 minutes after serum-starvation overnight or treated by Calyculin A (100 nM) at 37ºC for 30 minutes after serum-starvation overnight using GTX03203 SMAD2 (phospho Ser250) antibody [GT1291].
Dilution : 1:1000
Loading : 25μg per lane

WB analysis of C2C12 cells were treated by PMA/TPA (200 nM) at 37ºC for 30 minutes after serum-starvation overnight using GTX03203 SMAD2 (phospho Ser250) antibody [GT1291].
Dilution : 1:1000
Loading : 25μg per lane