SMAD2 (phospho Ser465 / Ser467) + SMAD3 (phospho Ser423 / Ser425) antibody [HL3379]

Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with SMAD2 (phospho Ser465 / Ser467) + SMAD3 (phospho Ser423 / Ser425) antibody [HL3379] (GTX641199) diluted at 1:1000 and SMAD2 / SMAD3 antibody (GTX111123) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with SMAD2 (phospho Ser465 / Ser467) + SMAD3 (phospho Ser423 / Ser425) antibody [HL3379] (GTX641199) diluted at 1:1000 and SMAD2 / SMAD3 antibody (GTX111123) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.