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SMAD2 (phospho Ser467) antibody

Anti-SMAD2 (phospho Ser467) antibody used in Western Blot (WB). GTX54987
Anti-SMAD2 (phospho Ser467) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54987
Anti-SMAD2 (phospho Ser467) antibody used in IHC (Paraffin sections) (IHC-P). GTX54987

Cat No. GTX54987

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

Human, Mouse, Rat
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
IHC-P 1:100 - 1:200
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of SMAD2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887908

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

SMAD family member 2 , JV18 , JV18-1 , MADH2 , MADR2 , hMAD-2 , hSMAD2

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, May 2012]

Database

Research Area

DATA IMAGES

Anti-SMAD2 (phospho Ser467) antibody used in Western Blot (WB). GTX54987

GTX54987 WB Image

WB analysis of human kidney (A), mouse brain (B), rat brain (C) whole cell lysates using GTX54987 SMAD2 (phospho Ser467) antibody.

Anti-SMAD2 (phospho Ser467) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54987

GTX54987 ICC/IF Image

ICC/IF analysis of formalin-fixed HeLa cells using GTX54987 SMAD2 (phospho Ser467) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

Anti-SMAD2 (phospho Ser467) antibody used in IHC (Paraffin sections) (IHC-P). GTX54987

GTX54987 IHC-P Image

IHC-P analysis of formalin fixed human lung cancer tissue section using GTX54987 SMAD2 (phospho Ser467) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

REFERENCE

REVIEW

SMAD2 (phospho Ser467) antibody Cat No. GTX54987

Rating ( Average 5 based on 1 users reviews)
Application
IHC (Frozen sections)(IHC-Fr) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Physiol Rep in 2017.PMID: 28292882
SDS
Glycerol.pdf
Sodium Azide.pdf