ChIP analysis of 2 million Jurkat cells treated with 50ng/ml of TGF-beta for one hour using GTX25487 SMAD2 (phospho Thr8) antibody. Normal Rabbit IgG was used as a negative IP control. The precipitated DNA was detected by PCR with primer set targeting to the promoter of ID1 gene as positive control and the inactive SAT2 used as negative control target.

WB (peptide competition) analysis of HepG2 cells stimulated with TGF beta using GTX25487 SMAD2 (phospho Thr8) antibody prior incubated with the non-phosphopeptide corresponding to the immunogen (Lane 2), a generic phosphothreonine containing peptide (Lane 3), or, the phosphopeptide immunogen (Lane 4) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with phosphatase (Lane 5) eliminates the signal further verifying that the antibody is phospho-specific.