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SMAD2 (phospho Thr8) antibody

Anti-SMAD2 (phospho Thr8) antibody used in Western Blot (WB). GTX52335

Cat No. GTX52335

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human, Mouse, Rat
Package
100 μg ($319)

APPLICATION

Application Note

WB 1:500-1:2000

Calculated MW

52 kDa. ( Note )

Specificity/Sensitivity

Smad2/3

PROPERTIES

Form

Liquid

Buffer

phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human Smad2/3 around the phosphorylation site of Thr8

Purification

Antigen affinity purification

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Smad Family Member 2 , Jv18 , Jv18-1 , Madh2 , Madr2 , Hmad-2 , Hsmad2 , Smad2

Background

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription re

Database

Research Area

DATA IMAGES

Anti-SMAD2 (phospho Thr8) antibody used in Western Blot (WB). GTX52335

GTX52335 WB Image

Western blot analysis of Smad2/3 phosphorylation expression in RAW264.7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf