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SMAD3 antibody [4C9]

Anti-SMAD3 antibody [4C9] used in IHC (Paraffin sections) (IHC-P). GTX34210
Anti-SMAD3 antibody [4C9] used in IHC (Paraffin sections) (IHC-P). GTX34210
Anti-SMAD3 antibody [4C9] used in Western Blot (WB). GTX34210

Cat No. GTX34210

Host Mouse
Clonality Monoclonal
Clone Name 4C9
Isotype IgG1
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Specificity/Sensitivity

Smad3 Mouse Monoclonal Antibody (4C9) detects endogenous levels of SMAD3
PROPERTIES

Form

Liquid

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Synthetic Peptide of Smad3

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Smad Family Member 3,Hspc193,Hst17436,Jv15-2,Lds1C,Lds3,Madh3,Smad3

Background

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq, Apr 2009]

Database

Research Area

DATA IMAGES
Anti-SMAD3 antibody [4C9] used in IHC (Paraffin sections) (IHC-P). GTX34210

GTX34210 IHC-P Image

Immunohistochemistry (IHC) analysis of paraffin-embedded Human Breast Carcinoma Tissue using Smad3 Mouse Monoclonal Antibody diluted at 1:200.

Anti-SMAD3 antibody [4C9] used in IHC (Paraffin sections) (IHC-P). GTX34210

GTX34210 IHC-P Image

Immunohistochemistry (IHC) analysis of paraffin-embedded Human Liver Carcinoma Tissue using Smad3 Mouse Monoclonal Antibody diluted at 1:200.

Anti-SMAD3 antibody [4C9] used in Western Blot (WB). GTX34210

GTX34210 WB Image

Western Blot (WB) analysis of 1) Mouse Brain Tissue Lysate, 2) Rat Brain Tissue Lysate using Smad3 Mouse Monoclonal Antibody diluted at 1:2000.