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SMAD3 antibody [5G11]

Anti-SMAD3 antibody [5G11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82809
Anti-SMAD3 antibody [5G11] used in Western Blot (WB). GTX82809

Cat. No. GTX82809

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5G11

Isotype

 IgG1

Application

 WB, ICC/IF, ELISA

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all SMAD3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
ELISA 1/10000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human SMAD3 expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SMAD family member 3 , HSPC193 , HsT17436 , JV15-2 , LDS1C , LDS3 , MADH3

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq, Apr 2009]

Database

Research Area

DATA IMAGES

Anti-SMAD3 antibody [5G11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82809

GTX82809 ICC/IF Image

ICC/IF analysis of NIH3T3 cells using GTX82809 SMAD3 antibody [5G11].
Green : SMAD3
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

Anti-SMAD3 antibody [5G11] used in Western Blot (WB). GTX82809

GTX82809 WB Image

WB analysis of A549 (1), HeLa (2), Jurkat (3), PC-2 (4) and NIH3T3 (5) cell lysate using GTX82809 SMAD3 antibody [5G11].

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399