*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
NIH-3T3 (starvation for 16 hr and 20 ng/ml TGF-β treatment for 30 min) , HeLa (300 ng/ml EGF and 1 ng/ml TGF-β treatment for 2 hr)
Rat, Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Carrier-protein conjugated synthetic peptide corresponding to residues around human SMAD3 (phospho Ser423/425). The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
SMAD family member 3 , HSPC193 , HsT17436 , JV15-2 , LDS1C , LDS3 , MADH3
Cytoplasm , Nucleus
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq]