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SMAD3 (phospho Ser423/425) antibody

Anti-SMAD3 (phospho Ser423/425) antibody used in Western Blot (WB). GTX129841
Anti-SMAD3 (phospho Ser423/425) antibody used in Western Blot (WB). GTX129841

Cat No. GTX129841

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

NIH-3T3 (starvation for 16 hr and 20 ng/ml TGF-β treatment for 30 min) , HeLa (300 ng/ml EGF and 1 ng/ml TGF-β treatment for 2 hr)

Predict Reactivity

Rat, Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide corresponding to residues around human SMAD3 (phospho Ser423/425). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

SMAD family member 3 , HSPC193 , HsT17436 , JV15-2 , LDS1C , LDS3 , MADH3

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SMAD3 (phospho Ser423/425) antibody used in Western Blot (WB). GTX129841

GTX129841 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SMAD3 (phospho Ser423/425) antibody (GTX129841) diluted at 1:500.

Anti-SMAD3 (phospho Ser423/425) antibody used in Western Blot (WB). GTX129841

GTX129841 WB Image

Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SMAD3 (phospho Ser423/425) antibody (GTX129841) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169