SMAD3 (phospho Ser423/Ser425) antibody [GT1207]

Name: SMAD3 (phospho Ser423/Ser425) antibody [GT1207]
Brand: GeneTex
SKU: GTX00969
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-SMAD3 (phospho Ser423/Ser425) antibody [GT1207] used in Western Blot (WB). GTX00969

Anti-SMAD3 (phospho Ser423/Ser425) antibody [GT1207] used in IHC (Paraffin sections) (IHC-P). GTX00969

Cat. No. GTX00969

Reference 4

Host	Rabbit
Clonality	Monoclonal
Clone Name	GT1207
Isotype	IgG
Application	WB, IHC-P
Reactivity	Human, Mouse, Rat
Package	100 μl ($399)

See all SMAD3 products

View product citations for antibody GTX00969 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500 - 1:2000
IHC-P	1:50 - 1:200

Not tested in other applications.

Calculated MW

48 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human Phospho-Smad3 (S423 + S425).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2885209

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SMAD family member 3 , HSPC193 , HsT17436 , JV152 , LDS1C , LDS3 , MADH3 , SMAD3

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq, Apr 2009]

Database

Gene ID: 4088 SMAD3
UniProt: P84022 SMAD3

Research Area

DATA IMAGES

GTX00969 WB Image

Untreated (–) and treated (+) A549 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SMAD3 (phospho Ser423/Ser425) antibody [GT1207] (GTX00969) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX00969 IHC-P Image

IHC-P analysis of rat brain tissue section using GTX00969 SMAD3 (phospho Ser423/Ser425) antibody [GT1207].
Dilution : 1:100

GTX00969 IHC-P Image

IHC-P analysis of mouse testis tissue section using GTX00969 SMAD3 (phospho Ser423/Ser425) antibody [GT1207].
Dilution : 1:100

GTX00969 WB Image

WB analysis of C6 cell extracts with/without PMA/TPA (200 nM, 37ºC, 10 min) treatment using GTX00969 SMAD3 (phospho Ser423/Ser425) antibody [GT1207].
Dilution : 1:1000
Loading : 25μg

GTX00969 IHC-P Image

IHC-P analysis of human esophageal tissue section using GTX00969 SMAD3 (phospho Ser423/Ser425) antibody [GT1207].
Dilution : 1:100

REFERENCE

Application Reference

Luo D et al. J Cell Mol Med 2023; 27 (3) : 456-469 Pirfenidone suppressed triple-negative breast cancer metastasis by inhibiting the activity of the TGF-β/SMAD pathway.

Application : WB

Reactivity : Human

Wenbiao Chen et al. Cell Commun Signal 2022; 20 (1) : 28 Ameliorating role of microRNA-378 carried by umbilical cord mesenchymal stem cells-released extracellular vesicles in mesangial proliferative glomerulonephritis.

Application : WB

Reactivity : Rat

Chen HY et al. Antioxidants (Basel) 2021; 10 (1) Characterization of TGF-β by Induced Oxidative Stress in Human Trabecular Meshwork Cells.

Application : WB

Reactivity : Human

Mar?n-Ramos NI et al. Circ Res 2020; 127 (9) : e210-e231 miR-18a Inhibits BMP4 and HIF-1α Normalizing Brain Arteriovenous Malformations.

Application : IHC-P

Reactivity : Human

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