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SMAD3 (phospho Ser423/Ser425) antibody [GT1207]

Anti-SMAD3 (phospho Ser423/Ser425) antibody [GT1207] used in Western Blot (WB). GTX00969
Anti-SMAD3 (phospho Ser423/Ser425) antibody [GT1207] used in Western Blot (WB). GTX00969
Anti-SMAD3 (phospho Ser423/Ser425) antibody [GT1207] used in IHC (Paraffin sections) (IHC-P). GTX00969
Anti-SMAD3 (phospho Ser423/Ser425) antibody [GT1207] used in IHC (Paraffin sections) (IHC-P). GTX00969
Anti-SMAD3 (phospho Ser423/Ser425) antibody [GT1207] used in IHC (Paraffin sections) (IHC-P). GTX00969

Cat No. GTX00969

Host

Rabbit

Clonality

Monoclonal

Clone Name

GT1207

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (pH7.3), 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human Phospho-Smad3 (S423 + S425).

Purification


Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2885209

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

SMAD family member 3 , HSPC193 , HsT17436 , JV152 , LDS1C , LDS3 , MADH3 , SMAD3

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq, Apr 2009]

Database

Research Area

DATA IMAGES

Anti-SMAD3 (phospho Ser423/Ser425) antibody [GT1207] used in Western Blot (WB). GTX00969

GTX00969 WB Image

Untreated (–) and treated (+) A549 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SMAD3 (phospho Ser423/Ser425) antibody [GT1207] (GTX00969) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SMAD3 (phospho Ser423/Ser425) antibody [GT1207] used in Western Blot (WB). GTX00969

GTX00969 WB Image

WB analysis of C6 cell extracts with/without PMA/TPA (200 nM, 37ºC, 10 min) treatment using GTX00969 SMAD3 (phospho Ser423/Ser425) antibody [GT1207].
Dilution : 1:1000
Loading : 25μg

Anti-SMAD3 (phospho Ser423/Ser425) antibody [GT1207] used in IHC (Paraffin sections) (IHC-P). GTX00969

GTX00969 IHC-P Image

IHC-P analysis of rat brain tissue section using GTX00969 SMAD3 (phospho Ser423/Ser425) antibody [GT1207].
Dilution : 1:100

Anti-SMAD3 (phospho Ser423/Ser425) antibody [GT1207] used in IHC (Paraffin sections) (IHC-P). GTX00969

GTX00969 IHC-P Image

IHC-P analysis of human esophageal tissue section using GTX00969 SMAD3 (phospho Ser423/Ser425) antibody [GT1207].
Dilution : 1:100

Anti-SMAD3 (phospho Ser423/Ser425) antibody [GT1207] used in IHC (Paraffin sections) (IHC-P). GTX00969

GTX00969 IHC-P Image

IHC-P analysis of mouse testis tissue section using GTX00969 SMAD3 (phospho Ser423/Ser425) antibody [GT1207].
Dilution : 1:100

REFERENCE

REVIEW

SMAD3 (phospho Ser423/Ser425) antibody [GT1207] Cat No. GTX00969

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 19-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : PC-3 cells
Amount used : 30 μg
Blocking : 5% non-fat milk in TBST, RT°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Lane Description :
Lane1: 24 h after irradiation with 0Gy
Lane2: 24 h after irradiation with 2Gy
Lane3: 24 h after irradiation with 4Gy
Lane4: 24 h after irradiation with 6Gy
Lane5: 24 h after irradiation with 8Gy
Lane6: 24 h after irradiation with 12Gy
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 369