Untreated (–) and treated (+) A549 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SMAD3 (phospho Ser423/Ser425) antibody [GT1207] (GTX00969) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

IHC-P analysis of human esophageal tissue section using GTX00969 SMAD3 (phospho Ser423/Ser425) antibody [GT1207].
Dilution : 1:100

WB analysis of C6 cell extracts with/without PMA/TPA (200 nM, 37ºC, 10 min) treatment using GTX00969 SMAD3 (phospho Ser423/Ser425) antibody [GT1207].
Dilution : 1:1000
Loading : 25μg

IHC-P analysis of mouse testis tissue section using GTX00969 SMAD3 (phospho Ser423/Ser425) antibody [GT1207].
Dilution : 1:100

IHC-P analysis of rat brain tissue section using GTX00969 SMAD3 (phospho Ser423/Ser425) antibody [GT1207].
Dilution : 1:100