GeneTex
United States (US)

SMAD3 (phospho Ser425) antibody

Anti-SMAD3 (phospho Ser425) antibody used in Western Blot (WB). GTX32335
Anti-SMAD3 (phospho Ser425) antibody used in IHC (Paraffin sections) (IHC-P). GTX32335

Cat No. GTX32335

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

48 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of SMAD3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Smad Family Member 3,Hspc193,Hst17436,Jv15-2,Lds1C,Lds3,Madh3,Smad3

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq, Apr 2009]

Database

Research Area

DATA IMAGES
Anti-SMAD3 (phospho Ser425) antibody used in Western Blot (WB). GTX32335

GTX32335 WB Image

WB analysis of EGF-treated A431 (A), HL60 TGF-treated (B) whole cell lysates using GTX32335 SMAD3 (phospho Ser425) antibody.

Anti-SMAD3 (phospho Ser425) antibody used in IHC (Paraffin sections) (IHC-P). GTX32335

GTX32335 IHC-P Image

IHC-P analysis of formalin fixed human breast cancer tissue section using GTX32335 SMAD3 (phospho Ser425) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)