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SMAD3 (phospho Ser425) antibody

Anti-SMAD3 (phospho Ser425) antibody used in Western Blot (WB). GTX50333
Anti-SMAD3 (phospho Ser425) antibody used in IHC (Paraffin sections) (IHC-P). GTX50333

Cat No. GTX50333

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of serine 425 (C-S-S-V-S(p)) derived from human SMAD3.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

SMAD family member 3 , HSPC193 , HsT17436 , JV15-2 , LDS1C , LDS3 , MADH3

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq, Apr 2009]

Database

Research Area

DATA IMAGES

Anti-SMAD3 (phospho Ser425) antibody used in Western Blot (WB). GTX50333

GTX50333 WB Image

WB analysis of extracts from HeLa cells untreated or treated with EGF using GTX50333 SMAD3 (phospho Ser425) antibody.

Anti-SMAD3 (phospho Ser425) antibody used in IHC (Paraffin sections) (IHC-P). GTX50333

GTX50333 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50333 SMAD3 (phospho Ser425) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

There are currently no references for SMAD3 (phospho Ser425) antibody (GTX50333). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for SMAD3 (phospho Ser425) antibody (GTX50333). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf