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SMAD3 (phospho Ser425) antibody

Anti-SMAD3 (phospho Ser425) antibody used in Western Blot (WB). GTX50333
Anti-SMAD3 (phospho Ser425) antibody used in IHC (Paraffin sections) (IHC-P). GTX50333

Cat. No. GTX50333

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all SMAD3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of serine 425 (C-S-S-V-S(p)) derived from human SMAD3.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SMAD family member 3 , HSPC193 , HsT17436 , JV15-2 , LDS1C , LDS3 , MADH3

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq, Apr 2009]

Database

Research Area

DATA IMAGES

Anti-SMAD3 (phospho Ser425) antibody used in Western Blot (WB). GTX50333

GTX50333 WB Image

WB analysis of extracts from HeLa cells untreated or treated with EGF using GTX50333 SMAD3 (phospho Ser425) antibody.

Anti-SMAD3 (phospho Ser425) antibody used in IHC (Paraffin sections) (IHC-P). GTX50333

GTX50333 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50333 SMAD3 (phospho Ser425) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399