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SMAD4 antibody [4G1C6]

Anti-SMAD4 antibody [4G1C6] used in IHC (Paraffin sections) (IHC-P). GTX82787
Anti-SMAD4 antibody [4G1C6] used in Flow cytometry (FACS). GTX82787
Anti-SMAD4 antibody [4G1C6] used in Western Blot (WB). GTX82787
Anti-SMAD4 antibody [4G1C6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82787

Cat No. GTX82787

Host Mouse
Clonality Monoclonal
Clone Name 4G1C6
Isotype IgG1
Application WB, ICC/IF, IHC-P, FACS, ELISA
Reactivity Human, Mouse
Package
100 μl ($319)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

60 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

Ascites, 0.03% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human SMAD4 expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Smad Family Member 4,Dpc4,Jip,Madh4,Myhrs,Smad4

Background

This gene encodes a member of the Smad family of signal transduction proteins. Smad proteins are phosphorylated and activated by transmembrane serine-threonine receptor kinases in response to TGF-beta signaling. The product of this gene forms homomeric complexes and heteromeric complexes with other activated Smad proteins, which then accumulate in the nucleus and regulate the transcription of target genes. This protein binds to DNA and recognizes an 8-bp palindromic sequence (GTCTAGAC) called the Smad-binding element (SBE). The Smad proteins are subject to complex regulation by post-translational modifications. Mutations or deletions in this gene have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome. [provided by RefSeq, Oct 2009]

Database

Research Area

DATA IMAGES
Anti-SMAD4 antibody [4G1C6] used in IHC (Paraffin sections) (IHC-P). GTX82787

GTX82787 IHC-P Image

IHC-P analysis of lung cancer tissue using GTX82787 SMAD4 antibody [4G1C6].

Anti-SMAD4 antibody [4G1C6] used in Flow cytometry (FACS). GTX82787

GTX82787 FACS Image

FACS analysis of K562 cells using GTX82787 SMAD4 antibody [4G1C6].
Green : SMAD4
Purple : negative control

Anti-SMAD4 antibody [4G1C6] used in Western Blot (WB). GTX82787

GTX82787 WB Image

WB analysis of A431 (1), SK-N-SH (2), K562 (3), HepG2 (4) and HUVE12 (5) cell lysate using GTX82787 SMAD4 antibody [4G1C6].

Anti-SMAD4 antibody [4G1C6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82787

GTX82787 ICC/IF Image

ICC/IF analysis of NIH3T3 cells using GTX82787 SMAD4 antibody [4G1C6].
Green : SMAD4
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

REFERENCE
There are currently no references for SMAD4 antibody [4G1C6] (GTX82787). Be the first to share your publications with this product.
REVIEW
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Package List Price ($)
$ 319