SMAD4 antibody [4G1C6]

Cat. No. GTX82787

Host	Mouse
Clonality	Monoclonal
Clone Name	4G1C6
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, FACS, ELISA
Reactivity	Human, Mouse
Package	100 μl ($399)

See all SMAD4 products

Datasheet
Document

View product citations for antibody GTX82787 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1/500 - 1/2000
ICC/IF	1/200 - 1/1000
IHC-P	1/200 - 1/1000
FACS	1/200 - 1/400
ELISA	1/10000

Not tested in other applications.

Calculated MW

60 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human SMAD4 expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SMAD family member 4 , DPC4 , JIP , MADH4 , MYHRS

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a member of the Smad family of signal transduction proteins. Smad proteins are phosphorylated and activated by transmembrane serine-threonine receptor kinases in response to TGF-beta signaling. The product of this gene forms homomeric complexes and heteromeric complexes with other activated Smad proteins, which then accumulate in the nucleus and regulate the transcription of target genes. This protein binds to DNA and recognizes an 8-bp palindromic sequence (GTCTAGAC) called the Smad-binding element (SBE). The Smad proteins are subject to complex regulation by post-translational modifications. Mutations or deletions in this gene have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome. [provided by RefSeq, Oct 2009]

Database

Gene ID: 4089 SMAD4
UniProt: Q13485 SMAD4

Research Area

DATA IMAGES

GTX82787 FACS Image

FACS analysis of K562 cells using GTX82787 SMAD4 antibody [4G1C6].
Green : SMAD4
Purple : negative control

GTX82787 WB Image

WB analysis of A431 (1), SK-N-SH (2), K562 (3), HepG2 (4) and HUVE12 (5) cell lysate using GTX82787 SMAD4 antibody [4G1C6].

GTX82787 IHC-P Image

IHC-P analysis of lung cancer tissue using GTX82787 SMAD4 antibody [4G1C6].

GTX82787 ICC/IF Image

ICC/IF analysis of NIH3T3 cells using GTX82787 SMAD4 antibody [4G1C6].
Green : SMAD4
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 399

Qty