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SMAD4 antibody

Anti-SMAD4 antibody used in IHC (Paraffin sections) (IHC-P). GTX03325

Cat. No. GTX03325

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 IHC-P

Reactivity

 Human
Package
500 μl ($399)
See all SMAD4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 1:50-1:100

Note :

IHC-P
Pretreatment : EDTA (pH 8.0) for 30 min at RT
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

Colon carcinoma, Pancreas carcinoma

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment corresponding to a region within amino acids 322 and 552 of SMAD4

Purification

Purified IgG

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SMAD family member 4 , DPC4 , JIP , MADH4 , MYHRS

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes a member of the Smad family of signal transduction proteins. Smad proteins are phosphorylated and activated by transmembrane serine-threonine receptor kinases in response to transforming growth factor (TGF)-beta signaling. The product of this gene forms homomeric complexes and heteromeric complexes with other activated Smad proteins, which then accumulate in the nucleus and regulate the transcription of target genes. This protein binds to DNA and recognizes an 8-bp palindromic sequence (GTCTAGAC) called the Smad-binding element (SBE). The protein acts as a tumor suppressor and inhibits epithelial cell proliferation. It may also have an inhibitory effect on tumors by reducing angiogenesis and increasng blood vessel hyperpermeability. The encoded protein is a crucial component of the bone morphogenetic protein signaling pathway. The Smad proteins are subject to complex regulation by post-translational modifications. Mutations or deletions in this gene have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-SMAD4 antibody used in IHC (Paraffin sections) (IHC-P). GTX03325

GTX03325 IHC-P Image

IHC-P analysis of human colon carcinoma tissue section using GTX03325 SMAD4 antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399