GeneTex
United States (US)

SMAD4 antibody

Comparison Overexpression
GTX112980_42368_20171110_WB_competitor_watermark_18121410_419.jpg
GTX112980_42368_20160325_IFA_18121410_458.jpg
GTX112980_42403_20160413_IHC-P_R_2_18121410_519.jpg
Genetex SMAD4 1 in 20000 small 400X 4.26.11_18121410_517.jpg
GTX112980_42172_20151015_WB_Merged_18121410_905.jpg
GTX112980_42599_20171121_IHC-P_R_18121410_954.jpg
GTX112980_42172_20150821_IHC-P_M_18121410_306.jpg
GTX112980_42599_20171121_IHC-P_M_18121410_346.jpg
GTX112980_42172_20150821_IHC-P_M_2_18121410_762.jpg
GTX112980_42599_20161019_IFA_18121410_943.jpg
GTX112980_42368_20181207_WB_B_19011519_855.jpg

Cat No. GTX112980

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2 , DDDDK-tagged SMAD4-transfected 293T

Predict Reactivity

Sheep, Bovine, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.55mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human SMAD4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DPC4 antibody, JIP antibody, MADH4 antibody, SMAD4 antibody, "mothers against decapentaplegic, Drosophila, homolog of, 4 antibody", deletion target in pancreatic carcinoma 4 antibody, MAD homolog 4 antibody, "SMAD, mothers against DPP homolog 4 antibody", mothers against decapentaplegic homolog 4 antibody, deleted in pancreatic carcinoma locus 4 antibody, SMAD family member 4 antibody

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes a member of the Smad family of signal transduction proteins. Smad proteins are phosphorylated and activated by transmembrane serine-threonine receptor kinases in response to TGF-beta signaling. The product of this gene forms homomeric complexes and heteromeric complexes with other activated Smad proteins, which then accumulate in the nucleus and regulate the transcription of target genes. This protein binds to DNA and recognizes an 8-bp palindromic sequence (GTCTAGAC) called the Smad-binding element (SBE). The Smad proteins are subject to complex regulation by post-translational modifications. Mutations or deletions in this gene have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX112980_42368_20171110_WB_competitor_watermark_18121410_419.jpg

GTX112980 WB Image

Comparison

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with SMAD4 antibody (GTX112980) diluted at 1:1000 and competitor's antibody (sc-7966) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX112980_42368_20160325_IFA_18121410_458.jpg

GTX112980 ICC/IF Image

SMAD4 antibody detects SMAD4 protein a tcytoplasm and nucleus by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SMAD4 protein stained by SMAD4 antibody (GTX112980) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX112980_42403_20160413_IHC-P_R_2_18121410_519.jpg

GTX112980 IHC-P Image

SMAD4 antibody detects SMAD4 protein at cytoplasm and nucleus in rat kidney by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
SMAD4 antibody (GTX112980) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Genetex SMAD4 1 in 20000 small 400X 4.26.11_18121410_517.jpg

GTX112980 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human ulcerative colitis tissue using SMAD4 antibody (GTX112980)

GTX112980_42172_20151015_WB_Merged_18121410_905.jpg

GTX112980 WB Image

SMAD4 antibody detects SMAD4 protein by western blot analysis. Various whole cell extracts were separated by 7.5% SDS-PAGE, and the membrane was blotted with SMAD4 antibody (GTX112980) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX112980_42599_20171121_IHC-P_R_18121410_954.jpg

GTX112980 IHC-P Image

SMAD4 antibody detects SMAD4 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
SMAD4 stained by SMAD4 antibody (GTX112980) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX112980_42172_20150821_IHC-P_M_18121410_306.jpg

GTX112980 IHC-P Image

SMAD4 antibody detects SMAD4 protein at cytoplasm and nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
SMAD4 antibody (GTX112980) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX112980_42599_20171121_IHC-P_M_18121410_346.jpg

GTX112980 IHC-P Image

SMAD4 antibody detects SMAD4 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
SMAD4 stained by SMAD4 antibody (GTX112980) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX112980_42172_20150821_IHC-P_M_2_18121410_762.jpg

GTX112980 IHC-P Image

SMAD4 antibody detects SMAD4 protein at cytoplasm and nucleus in mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
SMAD4 antibody (GTX112980) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX112980_42599_20161019_IFA_18121410_943.jpg

GTX112980 ICC/IF Image

SMAD4 antibody detects SMAD4 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SMAD4 protein stained by SMAD4 antibody (GTX112980) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX112980_42368_20181207_WB_B_19011519_855.jpg

GTX112980 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with SMAD4 antibody (GTX112980) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf