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SMAD5 antibody [3H9]

Anti-SMAD5 antibody [3H9] used in Flow cytometry (FACS). GTX60384
Anti-SMAD5 antibody [3H9] used in IHC (Paraffin sections) (IHC-P). GTX60384
Anti-SMAD5 antibody [3H9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60384
Anti-SMAD5 antibody [3H9] used in Western Blot (WB). GTX60384

Cat. No. GTX60384

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3H9

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

 Human, Rat
Package
100 μl ($399)
See all SMAD5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human SMAD5 expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SMAD family member 5 , DWFC , JV5-1 , MADH5

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]

Database

Research Area

DATA IMAGES

Anti-SMAD5 antibody [3H9] used in Flow cytometry (FACS). GTX60384

GTX60384 FACS Image

FACS analysis of Jurkat cells using GTX60384 SMAD5 antibody [3H9].
Green : SMAD5
Purple : negative control

Anti-SMAD5 antibody [3H9] used in IHC (Paraffin sections) (IHC-P). GTX60384

GTX60384 IHC-P Image

IHC-P analysis of human brain tissue (left) and lung cancer tissue (right) using GTX60384 SMAD5 antibody [3H9].

Anti-SMAD5 antibody [3H9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60384

GTX60384 ICC/IF Image

ICC/IF analysis of NTERA-2 cells using GTX60384 SMAD5 antibody [3H9].
Green : SMAD5
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

Anti-SMAD5 antibody [3H9] used in Western Blot (WB). GTX60384

GTX60384 WB Image

WB analysis of HeLa (1), SK-N-SH (2), PC-12 (3), Jurkat (4), and K562 (5) cell lysate using GTX60384 SMAD5 antibody [3H9].

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399