SMAD5 antibody [4B10-B10-B6]

Cat. No. GTX49219

Host	Mouse
Clonality	Monoclonal
Clone Name	4B10-B10-B6
Isotype	IgG1
Application	WB, ICC/IF, FACS
Reactivity	Human, Mouse
Package	100 μg ($399)

See all SMAD5 products

Datasheet
Document

View product citations for antibody GTX49219 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	Assay dependent
ICC/IF	Assay dependent
FACS	Assay dependent

Not tested in other applications.

Calculated MW

52 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.5% BSA, 50% glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant human SMAD5 protein fragments expressed in E.coli.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SMAD family member 5 , DWFC , JV5-1 , MADH5

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]

Database

Gene ID: 4090 SMAD5
UniProt: Q99717 SMAD5

Research Area

DATA IMAGES

GTX49219 FACS Image

FACS analysis of Jurkat cells using SMAD5 antibody at a dilution of 1:100 (red) or an isotype control (black).

GTX49219 ICC/IF Image

ICC/IF analysis of HeLa cells using SMAD5 antibody at a dilution of 1:75.

GTX49219 WB Image

WB analysis of mouse testis lysates using SMAD5 antibody at a dilution of 1:1,000.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μg

$ 399

Qty