GeneTex
United States (US)

SMAD5 antibody [N2C2], Internal

GTX105101_40464_IFA_19011506_285.jpg

Cat No. GTX105101

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

Target recombinant protein

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.9mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SMAD5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DKFZp781C1895 antibody, DKFZp781O1323 antibody, Dwfc antibody, JV5-1 antibody, MADH5 antibody, SMAD5 antibody, "MAD, mothers against decapentaplegic homolog 5 antibody", mothers against decapentaplegic homolog 5 antibody, "SMAD, mothers against DPP homolog 5 antibody", "mothers against decapentaplegic, drosophila, homolog of, 5 antibody", SMA- and MAD-related protein 5 antibody, SMAD family member 5 antibody

Cellular Localization

Cytoplasm , Nucleus

Database

Research Area

DATA IMAGES
GTX105101_40464_IFA_19011506_285.jpg

GTX105101 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using SMAD5(GTX105101) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf