APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
Target recombinant protein
Predict Reactivity
Mouse, Rat, Bovine, Chicken, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.9mg/ml(Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human SMAD5. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
DKFZp781C1895 antibody, DKFZp781O1323 antibody, Dwfc antibody, JV5-1 antibody, MADH5 antibody, SMAD5 antibody, "MAD, mothers against decapentaplegic homolog 5 antibody", mothers against decapentaplegic homolog 5 antibody, "SMAD, mothers against DPP homolog 5 antibody", "mothers against decapentaplegic, drosophila, homolog of, 5 antibody", SMA- and MAD-related protein 5 antibody, SMAD family member 5 antibody
Cellular Localization
Cytoplasm , Nucleus
Database
Research Area
DATA IMAGES
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GTX105101 ICC/IF Image
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using SMAD5(GTX105101) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
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