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SMAD5 antibody

Anti-SMAD5 antibody used in Western Blot (WB). GTX108917
Anti-SMAD5 antibody used in Western Blot (WB). GTX108917
Anti-SMAD5 antibody used in Western Blot (WB). GTX108917
Anti-SMAD5 antibody used in Western Blot (WB). GTX108917
Anti-SMAD5 antibody used in IHC (Paraffin sections) (IHC-P). GTX108917

Cat. No. GTX108917

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all SMAD5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

HeLa , Raw264.7 , C2C12 , Rat2

Predict Reactivity

Drosophila, Bovine, Chicken, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SMAD5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10721793

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SMAD family member 5 , DWFC , JV5-1 , MADH5

Cellular Localization

Cytoplasm , Nucleus

Database

Research Area

DATA IMAGES

Anti-SMAD5 antibody used in Western Blot (WB). GTX108917

GTX108917 WB Image

SMAD5 antibody detects SMAD5 protein by Western blot analysis.
A. 30 μg Rat2 whole cell lysate/extract
10 % SDS-PAGE
SMAD5 antibody (GTX108917) dilution: 1:1000

Anti-SMAD5 antibody used in Western Blot (WB). GTX108917

GTX108917 WB Image

Sample (30 ug of whole cell lysate)
A: HeLa
10% SDS PAGE
GTX108917 diluted at 1:1000

Anti-SMAD5 antibody used in Western Blot (WB). GTX108917

GTX108917 WB Image

SMAD5 antibody detects SMAD5 protein by Western blot analysis.
A. 30 μg Raw264.7 whole cell lysate/extract
B. 30 μg C2C12 whole cell lysate/extract
10 % SDS-PAGE
SMAD5 antibody (GTX108917) dilution: 1:1000

Anti-SMAD5 antibody used in Western Blot (WB). GTX108917

GTX108917 WB Image

SMAD5 antibody detects smad5 protein by western blot analysis. Various zebrafish tissue extracts (30 μg) were separated by 10 % SDS-PAGE, and the membrane was blotted with SMAD5 antibody (GTX108917) at a dilution of 1:1000.

Anti-SMAD5 antibody used in IHC (Paraffin sections) (IHC-P). GTX108917

GTX108917 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using SMAD5 antibody (GTX108917) at 1:300 dilution.

REFERENCE

Application Reference

Yang G et al. J Biol Chem 2014; 289 (45) : 31492-502 An Atypical Canonical Bone Morphogenetic Protein (BMP) Signaling Pathway Regulates Msh Homeobox 1 (Msx1) Expression during Odontogenesis.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169