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SMAD5 antibody

Anti-SMAD5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32881
Anti-SMAD5 antibody used in IHC (Paraffin sections) (IHC-P). GTX32881
Anti-SMAD5 antibody used in Western Blot (WB). GTX32881

Cat. No. GTX32881

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all SMAD5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 186-465 of human SMAD5 (NP_001001420.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SMAD family member 5 , DWFC , JV5-1 , MADH5

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]

Database

Research Area

DATA IMAGES

Anti-SMAD5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32881

GTX32881 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX32881 SMAD5 antibody.
Blue : DAPI
Dilution : 1:100

Anti-SMAD5 antibody used in IHC (Paraffin sections) (IHC-P). GTX32881

GTX32881 IHC-P Image

IHC-P analysis of human colon tissue using GTX32881 SMAD5 antibody.
Dilution : 1:150

Anti-SMAD5 antibody used in Western Blot (WB). GTX32881

GTX32881 WB Image

WB analysis of various sample lysates using GTX32881 SMAD5 antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399