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SMAD5 antibody

Anti-SMAD5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32881
Anti-SMAD5 antibody used in Western Blot (WB). GTX32881
Anti-SMAD5 antibody used in IHC (Paraffin sections) (IHC-P). GTX32881

Cat No. GTX32881

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ICC/IF 1:50-1:200
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 186-465 of human SMAD5 (NP_001001420.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

SMAD family member 5 , DWFC , JV5-1 , MADH5

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]

Database

Research Area

DATA IMAGES

Anti-SMAD5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32881

GTX32881 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX32881 SMAD5 antibody.
Blue : DAPI
Dilution : 1:100

Anti-SMAD5 antibody used in Western Blot (WB). GTX32881

GTX32881 WB Image

WB analysis of various sample lysates using GTX32881 SMAD5 antibody.
Loading : 25ug per lane
Dilution : 1:1000

Anti-SMAD5 antibody used in IHC (Paraffin sections) (IHC-P). GTX32881

GTX32881 IHC-P Image

IHC-P analysis of human lung tissue using GTX32881 SMAD5 antibody.
Dilution : 1:100

REFERENCE

There are currently no references for SMAD5 antibody (GTX32881). Be the first to share your publications with this product.

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 329