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SMAD5 (phospho Ser463/Ser465) antibody [SY09-03]

Anti-SMAD5 (phospho Ser463/Ser465) antibody [SY09-03] used in Western Blot (WB). GTX01450
Anti-SMAD5 (phospho Ser463/Ser465) antibody [SY09-03] used in Western Blot (WB). GTX01450
Anti-SMAD5 (phospho Ser463/Ser465) antibody [SY09-03] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01450
Anti-SMAD5 (phospho Ser463/Ser465) antibody [SY09-03] used in IHC (Paraffin sections) (IHC-P). GTX01450
Anti-SMAD5 (phospho Ser463/Ser465) antibody [SY09-03] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01450
Anti-SMAD5 (phospho Ser463/Ser465) antibody [SY09-03] used in IHC (Paraffin sections) (IHC-P). GTX01450
Anti-SMAD5 (phospho Ser463/Ser465) antibody [SY09-03] used in IHC (Paraffin sections) (IHC-P). GTX01450

Cat No. GTX01450

Host

Rabbit

Clonality

Monoclonal

Clone Name

SY09-03

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:2000
ICC/IF 1:50-1:200
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

1x TBS (pH7.4), 1% BSA, 40% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

SMAD family member 5 , DWFC , JV51 , MADH5 , SMAD5

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]

Database

Research Area

DATA IMAGES

Anti-SMAD5 (phospho Ser463/Ser465) antibody [SY09-03] used in Western Blot (WB). GTX01450

GTX01450 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SMAD5 (phospho Ser463/Ser465) antibody [SY09-03] (GTX01450) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SMAD5 (phospho Ser463/Ser465) antibody [SY09-03] used in Western Blot (WB). GTX01450

GTX01450 WB Image

Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SMAD5 (phospho Ser463/Ser465) antibody [SY09-03] (GTX01450) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SMAD5 (phospho Ser463/Ser465) antibody [SY09-03] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01450

GTX01450 ICC/IF Image

ICC/IF analysis of PFA fixed SKOV-3 cells using GTX01450 SMAD5 (phospho Ser463/Ser465) antibody [SY09-03].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

Anti-SMAD5 (phospho Ser463/Ser465) antibody [SY09-03] used in IHC (Paraffin sections) (IHC-P). GTX01450

GTX01450 IHC-P Image

IHC-P analysis of human tonsil tissue section using GTX01450 SMAD5 (phospho Ser463/Ser465) antibody [SY09-03].

Anti-SMAD5 (phospho Ser463/Ser465) antibody [SY09-03] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01450

GTX01450 ICC/IF Image

ICC/IF analysis of PFA fixed HepG2 cells using GTX01450 SMAD5 (phospho Ser463/Ser465) antibody [SY09-03].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

Anti-SMAD5 (phospho Ser463/Ser465) antibody [SY09-03] used in IHC (Paraffin sections) (IHC-P). GTX01450

GTX01450 IHC-P Image

IHC-P analysis of human breast carcinoma tissue section using GTX01450 SMAD5 (phospho Ser463/Ser465) antibody [SY09-03].

Anti-SMAD5 (phospho Ser463/Ser465) antibody [SY09-03] used in IHC (Paraffin sections) (IHC-P). GTX01450

GTX01450 IHC-P Image

IHC-P analysis of mouse brain tissue section using GTX01450 SMAD5 (phospho Ser463/Ser465) antibody [SY09-03].

REFERENCE

There are currently no references for SMAD5 (phospho Ser463/Ser465) antibody [SY09-03] (GTX01450). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for SMAD5 (phospho Ser463/Ser465) antibody [SY09-03] (GTX01450). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 369