SMAD5 (phospho Ser463/Ser465) antibody [SY09-03]

Cat No. GTX01450

Host	Rabbit
Clonality	Monoclonal
Clone Name	SY09-03
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse
Package	100 μl ($369)

See all SMAD5 products

Datasheet
Document

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:1000-1:2000
ICC/IF	1:50-1:200
IHC-P	1:50-1:200

Not tested in other applications.

Calculated MW

52 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

1x TBS (pH7.4), 1% BSA, 40% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

SMAD family member 5 , DWFC , JV51 , MADH5 , SMAD5

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]

Database

Gene ID: 4090 SMAD5
UniProt: Q99717 SMAD5

Research Area

DATA IMAGES

GTX01450 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SMAD5 (phospho Ser463/Ser465) antibody [SY09-03] (GTX01450) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX01450 WB Image

Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SMAD5 (phospho Ser463/Ser465) antibody [SY09-03] (GTX01450) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX01450 ICC/IF Image

ICC/IF analysis of PFA fixed SKOV-3 cells using GTX01450 SMAD5 (phospho Ser463/Ser465) antibody [SY09-03].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

GTX01450 IHC-P Image

IHC-P analysis of human tonsil tissue section using GTX01450 SMAD5 (phospho Ser463/Ser465) antibody [SY09-03].

GTX01450 ICC/IF Image

ICC/IF analysis of PFA fixed HepG2 cells using GTX01450 SMAD5 (phospho Ser463/Ser465) antibody [SY09-03].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

GTX01450 IHC-P Image

IHC-P analysis of human breast carcinoma tissue section using GTX01450 SMAD5 (phospho Ser463/Ser465) antibody [SY09-03].

GTX01450 IHC-P Image

IHC-P analysis of mouse brain tissue section using GTX01450 SMAD5 (phospho Ser463/Ser465) antibody [SY09-03].

REFERENCE

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REVIEW

There are currently no reviews for SMAD5 (phospho Ser463/Ser465) antibody [SY09-03] (GTX01450). Be the first to share your experience with this product.

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SDS
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 369

Qty

SMAD5 (phospho Ser463/Ser465) antibody [SY09-03]

Cat No. GTX01450

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX01450 WB Image

GTX01450 WB Image

GTX01450 ICC/IF Image

GTX01450 IHC-P Image

GTX01450 ICC/IF Image

GTX01450 IHC-P Image

GTX01450 IHC-P Image

REFERENCE

REVIEW

Product

Research

Support

Contact

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.