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SMAD6 antibody

Anti-SMAD6 antibody used in Western Blot (WB). GTX13727
Anti-SMAD6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX13727
Anti-SMAD6 antibody used in Western Blot (WB). GTX13727

Cat. No. GTX13727

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Sheep, Primate
Package
100 μg ($409)
See all SMAD6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5 - 2 μg/ml
ICC/IF 1 - 5 μg/ml
IHC-P 5 - 10 μg/ml
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Predict Reactivity

Horse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

This antibody was developed against 2 synthetic peptides found between amino acids 30-150 and 300-400 of human SMAD6 (GenBank Accession No. AAH12986.1). These peptide sequences are specific to SMAD6, and have high homology to SMAD6 in multiple species.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SMAD family member 6 , AOVD2 , HsT17432 , MADH6 , MADH7

Cellular Localization

Nucleus

Background

The protein encoded by this gene belongs to the SMAD family of proteins, which are related to Drosophila 'mothers against decapentaplegic' (Mad) and C. elegans Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions in the negative regulation of BMP and TGF-beta/activin-signalling. Multiple transcript variants encoding different isoforms have been found for this gene.

Database

Research Area

DATA IMAGES

Anti-SMAD6 antibody used in Western Blot (WB). GTX13727

GTX13727 WB Image

WB analysis of HepG2 cell lysate using GTX13727 SMAD6 antibody.

Anti-SMAD6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX13727

GTX13727 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX13727 SMAD6 antibody.
Green : primary antibody
Red : Tubulin
Blue : DAPI
Dilution : 1:100
Fixation : 10% formalin
Permibilization : 0.5% Triton-X100

Anti-SMAD6 antibody used in Western Blot (WB). GTX13727

GTX13727 WB Image

WB analysis of Jurkat cell lysate using GTX13727 SMAD6 antibody.
Dilution : 2 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409