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SMAD9 antibody, Internal

Anti-SMAD9 antibody, Internal used in Western Blot (WB). GTX89360
Anti-SMAD9 antibody, Internal used in ELISA (ELISA). GTX89360
Anti-SMAD9 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89360

Cat. No. GTX89360

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human
Package
100 μg ($399)
See all SMAD9 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.3-1μg/ml
IHC-P 5μg/ml
ELISA Assay dependent
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89360-PEP).

Product Note

This antibody is not expected to cross-react with SMAD1 and SMAD5.

Predict Reactivity

Mouse, Rat, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-HSEPLMPHNATYPD, from the internal region of the protein sequence according to NP_005896.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SMAD family member 9 , MADH6 , MADH9 , PPH2 , SMAD8 , SMAD8/9 , SMAD8A , SMAD8B

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene is a member of the SMAD family, which transduces signals from TGF-beta family members. The encoded protein is activated by bone morphogenetic proteins and interacts with SMAD4. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2010]

Database

Research Area

DATA IMAGES

Anti-SMAD9 antibody, Internal used in Western Blot (WB). GTX89360

GTX89360 WB Image

WB analysis of human skin lysate using GTX89360 SMAD9 antibody, Internal.
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer

Anti-SMAD9 antibody, Internal used in ELISA (ELISA). GTX89360

GTX89360 ELISA Image

ELISA analysis of SMAD9 protein using GTX89360 SMAD9 antibody, Internal. This antibody was used as a detecting antibody.
Dilution : 1.5μg/ml

Anti-SMAD9 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89360

GTX89360 IHC-P Image

IHC-P analysis of human kidney using GTX89360 SMAD9 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 5μg/ml

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399