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SMAD9 antibody

Anti-SMAD9 antibody used in Western Blot (WB). GTX114540
Anti-SMAD9 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114540
Anti-SMAD9 antibody used in IHC (Paraffin sections) (IHC-P). GTX114540

Cat. No. GTX114540

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all SMAD9 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.68 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SMAD9. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620458

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SMAD family member 9 , MADH6 , MADH9 , PPH2 , SMAD8 , SMAD8/9 , SMAD8A , SMAD8B

Cellular Localization

Cytoplasm , Nucleus

Background

SMAD family members, such as SMAD9, transduce signals from members of the TGF-beta (see TGFB1; MIM 190180) superfamily, which regulate growth, differentiation, apoptosis, and development (Nishita et al., 1999 [PubMed 10583507]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-SMAD9 antibody used in Western Blot (WB). GTX114540

GTX114540 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with SMAD9 antibody (GTX114540) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SMAD9 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114540

GTX114540 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using SMAD9(GTX114540) antibody at 1:500 dilution.

Anti-SMAD9 antibody used in IHC (Paraffin sections) (IHC-P). GTX114540

GTX114540 IHC-P Image

SMAD9 antibody detects SMAD9 protein at cytoplasm on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
SMAD9 antibody (GTX114540) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169