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SMAD9 antibody

Anti-SMAD9 antibody used in IHC (Paraffin sections) (IHC-P). GTX114540
Anti-SMAD9 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114540
Anti-SMAD9 antibody used in Western Blot (WB). GTX114540

Cat No. GTX114540

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

NT2D1

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.68 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SMAD9. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620458

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

SMAD family member 9 , MADH6 , MADH9 , PPH2 , SMAD8 , SMAD8/9 , SMAD8A , SMAD8B

Cellular Localization

Cytoplasm , Nucleus

Background

SMAD family members, such as SMAD9, transduce signals from members of the TGF-beta (see TGFB1; MIM 190180) superfamily, which regulate growth, differentiation, apoptosis, and development (Nishita et al., 1999 [PubMed 10583507]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-SMAD9 antibody used in IHC (Paraffin sections) (IHC-P). GTX114540

GTX114540 IHC-P Image

SMAD9 antibody detects SMAD9 protein at cytoplasm on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
SMAD9 antibody (GTX114540) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SMAD9 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114540

GTX114540 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using SMAD9(GTX114540) antibody at 1:500 dilution.

Anti-SMAD9 antibody used in Western Blot (WB). GTX114540

GTX114540 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with SMAD9 antibody (GTX114540) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169