GeneTex
United States (US)

SMAD9 antibody

GTX64515_20180530_ICCIF_18121411_923.jpg
GTX64515_20180530_IHC-P_1_18121411_979.jpg
GTX64515_20180530_IHC-P_5_18121411_329.jpg
GTX64515_20180530_IHC-P_4_18121411_155.jpg
GTX64515_20180530_WB_18121411_532.jpg
GTX64515_20180530_IHC-P_3_18121411_742.jpg
GTX64515_20180530_IHC-P_2_18121411_996.jpg
GTX64515_20180530_IHC-P_18121411_984.jpg

Cat No. GTX64515

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

52 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant funsion protein containing a sequence corresponding to amino acids 1-300 of human SMAD9 (NP_005896.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

MADH6 Antibody , SMAD8B Antibody , SMAD8 Antibody , MADH9 Antibody , SMAD8A Antibody

Background

The protein encoded by this gene is a member of the SMAD family, which transduces signals from TGF-beta family members. The encoded protein is activated by bone morphogenetic proteins and interacts with SMAD4. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2010]

Database

Research Area

DATA IMAGES
GTX64515_20180530_ICCIF_18121411_923.jpg

GTX64515 ICC/IF Image

ICC/IF analysis of A549 cell using GTX64515 SMAD9 antibody.
Blue : DAPI

GTX64515_20180530_IHC-P_1_18121411_979.jpg

GTX64515 IHC-P Image

IHC-P analysis of human kidney cancer tissue using GTX64515 SMAD9 antibody.
Dilution : 1:100

GTX64515_20180530_IHC-P_5_18121411_329.jpg

GTX64515 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX64515 SMAD9 antibody.
Dilution : 1:100

GTX64515_20180530_IHC-P_4_18121411_155.jpg

GTX64515 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX64515 SMAD9 antibody.
Dilution : 1:100

GTX64515_20180530_WB_18121411_532.jpg

GTX64515 WB Image

WB analysis of various samples using GTX64515 SMAD9 antibody.
Dilution : 1:1000
Loading : 25μg per lane

GTX64515_20180530_IHC-P_3_18121411_742.jpg

GTX64515 IHC-P Image

IHC-P analysis of human liver injury tissue using GTX64515 SMAD9 antibody.
Dilution : 1:100

GTX64515_20180530_IHC-P_2_18121411_996.jpg

GTX64515 IHC-P Image

IHC-P analysis of human kidney tissue using GTX64515 SMAD9 antibody.
Dilution : 1:100

GTX64515_20180530_IHC-P_18121411_984.jpg

GTX64515 IHC-P Image

IHC-P analysis of human brain cancer tissue using GTX64515 SMAD9 antibody.
Dilution : 1:100