APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Bovine, Chicken(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human SMARCA2. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2909972
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2 , BAF190 , BRM , NCBRS , SNF2 , SNF2L2 , SNF2LA , SWI2 , Sth1p , hBRM , hSNF2a
Cellular Localization
Nucleus
Background
The protein encoded by this gene is a member of the SWI/SNF family of proteins and is highly similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene, which contains a trinucleotide repeat (CAG) length polymorphism. [provided by RefSeq, Jan 2014]
Database
Research Area
DATA IMAGES
![Anti-SMARCA2 antibody [HL1115] used in Western Blot (WB). GTX636330](/templates/default/js/lightbox/images/loading.gif "Anti-SMARCA2 antibody [HL1115] used in Western Blot (WB). GTX636330")
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GTX636330 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with SMARCA2 antibody [HL1115] (GTX636330) diluted at 1:1000 and competitor's antibody (Competitor) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX636330 ICC/IF Image
SMARCA2 antibody [HL1115] detects SMARCA2 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SMARCA2 stained by SMARCA2 antibody [HL1115] (GTX636330) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
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GTX636330 IHC-P Image
SMARCA2 antibody [HL1115] detects SMARCA2 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.SMARCA2 stained by SMARCA2 antibody [HL1115] (GTX636330) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636330 IHC-P Image
SMARCA2 antibody [HL1115] detects SMARCA2 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.SMARCA2 stained by SMARCA2 antibody [HL1115] (GTX636330) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636330 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SMARCA2 antibody [HL1115] (GTX636330) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX636330 IHC-P Image
SMARCA2 antibody [HL1115] detects SMARCA2 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissue.SMARCA2 stained by SMARCA2 antibody [HL1115] (GTX636330) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636330 IHC-P Image
SMARCA2 antibody [HL1115] detects SMARCA2 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissue.SMARCA2 stained by SMARCA2 antibody [HL1115] (GTX636330) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
There are currently no references for SMARCA2 antibody [HL1115] (GTX636330). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for SMARCA2 antibody [HL1115] (GTX636330). Be the first to share your experience with this product.
![Anti-SMARCA2 antibody [HL1115] used in Western Blot (WB). GTX636330](/upload/website/prouct_img/small/GTX636330/GTX636330_44438_20220812_WB_competitor_watermark_22081423_322.webp "Anti-SMARCA2 antibody [HL1115] used in Western Blot (WB). GTX636330")
![Anti-SMARCA2 antibody [HL1115] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636330](/upload/website/prouct_img/small/GTX636330/GTX636330_44438_20211224_ICC_IF_w_23061202_116.webp "Anti-SMARCA2 antibody [HL1115] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636330")
![Anti-SMARCA2 antibody [HL1115] used in IHC (Paraffin sections) (IHC-P). GTX636330](/upload/website/prouct_img/small/GTX636330/GTX636330_44438_20220304_IHC-P_R_1_w_23061202_992.webp "Anti-SMARCA2 antibody [HL1115] used in IHC (Paraffin sections) (IHC-P). GTX636330")
![Anti-SMARCA2 antibody [HL1115] used in IHC (Paraffin sections) (IHC-P). GTX636330](/upload/website/prouct_img/small/GTX636330/GTX636330_44438_20211022_IHC-P_M_w_23061202_165.webp "Anti-SMARCA2 antibody [HL1115] used in IHC (Paraffin sections) (IHC-P). GTX636330")
![Anti-SMARCA2 antibody [HL1115] used in Western Blot (WB). GTX636330](/upload/website/prouct_img/small/GTX636330/GTX636330_44438_20230707_WB_TPM_watermark_23071223_195.webp "Anti-SMARCA2 antibody [HL1115] used in Western Blot (WB). GTX636330")
![Anti-SMARCA2 antibody [HL1115] used in IHC (Paraffin sections) (IHC-P). GTX636330](/upload/website/prouct_img/small/GTX636330/GTX636330_44837_20221228_IHC-P_multiple_M_22122901_511.webp "Anti-SMARCA2 antibody [HL1115] used in IHC (Paraffin sections) (IHC-P). GTX636330")
![Anti-SMARCA2 antibody [HL1115] used in IHC (Paraffin sections) (IHC-P). GTX636330](/upload/website/prouct_img/small/GTX636330/GTX636330_44837_20221228_IHC-P_multiple_R_22122901_824.webp "Anti-SMARCA2 antibody [HL1115] used in IHC (Paraffin sections) (IHC-P). GTX636330")
![Anti-SMARCA2 antibody [HL1115] used in Western Blot (WB). GTX636330](/upload/website/prouct_img/normal/GTX636330/GTX636330_44438_20220812_WB_competitor_watermark_22081423_322.webp "Anti-SMARCA2 antibody [HL1115] used in Western Blot (WB). GTX636330")
![Anti-SMARCA2 antibody [HL1115] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636330](/upload/website/prouct_img/normal/GTX636330/GTX636330_44438_20211224_ICC_IF_w_23061202_116.webp "Anti-SMARCA2 antibody [HL1115] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636330")
![Anti-SMARCA2 antibody [HL1115] used in IHC (Paraffin sections) (IHC-P). GTX636330](/upload/website/prouct_img/normal/GTX636330/GTX636330_44438_20220304_IHC-P_R_1_w_23061202_992.webp "Anti-SMARCA2 antibody [HL1115] used in IHC (Paraffin sections) (IHC-P). GTX636330")
![Anti-SMARCA2 antibody [HL1115] used in IHC (Paraffin sections) (IHC-P). GTX636330](/upload/website/prouct_img/normal/GTX636330/GTX636330_44438_20211022_IHC-P_M_w_23061202_165.webp "Anti-SMARCA2 antibody [HL1115] used in IHC (Paraffin sections) (IHC-P). GTX636330")
![Anti-SMARCA2 antibody [HL1115] used in Western Blot (WB). GTX636330](/upload/website/prouct_img/normal/GTX636330/GTX636330_44438_20230707_WB_TPM_watermark_23071223_195.webp "Anti-SMARCA2 antibody [HL1115] used in Western Blot (WB). GTX636330")
![Anti-SMARCA2 antibody [HL1115] used in IHC (Paraffin sections) (IHC-P). GTX636330](/upload/website/prouct_img/normal/GTX636330/GTX636330_44837_20221228_IHC-P_multiple_M_22122901_511.webp "Anti-SMARCA2 antibody [HL1115] used in IHC (Paraffin sections) (IHC-P). GTX636330")
![Anti-SMARCA2 antibody [HL1115] used in IHC (Paraffin sections) (IHC-P). GTX636330](/upload/website/prouct_img/normal/GTX636330/GTX636330_44837_20221228_IHC-P_multiple_R_22122901_824.webp "Anti-SMARCA2 antibody [HL1115] used in IHC (Paraffin sections) (IHC-P). GTX636330")