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SMARCA2 antibody [HL1115]

Anti-SMARCA2 antibody [HL1115] used in Western Blot (WB). GTX636330
Anti-SMARCA2 antibody [HL1115] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636330
Anti-SMARCA2 antibody [HL1115] used in IHC (Paraffin sections) (IHC-P). GTX636330
Anti-SMARCA2 antibody [HL1115] used in IHC (Paraffin sections) (IHC-P). GTX636330
Anti-SMARCA2 antibody [HL1115] used in IHC (Paraffin sections) (IHC-P). GTX636330
Anti-SMARCA2 antibody [HL1115] used in IHC (Paraffin sections) (IHC-P). GTX636330
Anti-SMARCA2 antibody [HL1115] used in Western Blot (WB). GTX636330

Cat. No. GTX636330

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1115

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

181 kDa. ( Note )

Predict Reactivity

Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human SMARCA2. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2909972

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2 , BAF190 , BRM , NCBRS , SNF2 , SNF2L2 , SNF2LA , SWI2 , Sth1p , hBRM , hSNF2a

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a member of the SWI/SNF family of proteins and is highly similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene, which contains a trinucleotide repeat (CAG) length polymorphism. [provided by RefSeq, Jan 2014]

Database

Research Area

DATA IMAGES

Anti-SMARCA2 antibody [HL1115] used in Western Blot (WB). GTX636330

GTX636330 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with SMARCA2 antibody [HL1115] (GTX636330) diluted at 1:1000 and competitor's antibody (Competitor) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-SMARCA2 antibody [HL1115] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636330

GTX636330 ICC/IF Image

SMARCA2 antibody [HL1115] detects SMARCA2 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SMARCA2 stained by SMARCA2 antibody [HL1115] (GTX636330) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Anti-SMARCA2 antibody [HL1115] used in IHC (Paraffin sections) (IHC-P). GTX636330

GTX636330 IHC-P Image

SMARCA2 antibody [HL1115] detects SMARCA2 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
SMARCA2 stained by SMARCA2 antibody [HL1115] (GTX636330) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SMARCA2 antibody [HL1115] used in IHC (Paraffin sections) (IHC-P). GTX636330

GTX636330 IHC-P Image

SMARCA2 antibody [HL1115] detects SMARCA2 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
SMARCA2 stained by SMARCA2 antibody [HL1115] (GTX636330) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SMARCA2 antibody [HL1115] used in IHC (Paraffin sections) (IHC-P). GTX636330

GTX636330 IHC-P Image

SMARCA2 antibody [HL1115] detects SMARCA2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissue.
SMARCA2 stained by SMARCA2 antibody [HL1115] (GTX636330) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SMARCA2 antibody [HL1115] used in IHC (Paraffin sections) (IHC-P). GTX636330

GTX636330 IHC-P Image

SMARCA2 antibody [HL1115] detects SMARCA2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissue.
SMARCA2 stained by SMARCA2 antibody [HL1115] (GTX636330) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SMARCA2 antibody [HL1115] used in Western Blot (WB). GTX636330

GTX636330 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SMARCA2 antibody [HL1115] (GTX636330) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

There are currently no references for SMARCA2 antibody [HL1115] (GTX636330). Be the first to share your publications with this product.

REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations