APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa
Predict Reactivity
Bovine, Chicken(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.89 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human SMARCA2. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2887080
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2 , BAF190 , BRM , NCBRS , SNF2 , SNF2L2 , SNF2LA , SWI2 , Sth1p , hBRM , hSNF2a
Cellular Localization
Nucleus
Background
The protein encoded by this gene is a member of the SWI/SNF family of proteins and is highly similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. Two transcript variants encoding different isoforms have been found for this gene, which contains a trinucleotide repeat (CAG) length polymorphism. [provided by RefSeq]
Database
Research Area
DATA IMAGES
|
GTX133724 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with SMARCA2 antibody (GTX133724) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
|
|
|
GTX133724 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with SMARCA2 antibody (GTX133724) diluted at 1:1000 and competitor's antibody (sc-6450) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
|
|
|
GTX133724 ICC/IF Image
SMARCA2 antibody detects SMARCA2 protein at nucleolus by immunofluorescent analysis.Sample: DIV10 rat hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SMARCA2 stained by SMARCA2 antibody (GTX133724) diluted at 1:500.Red: Tau, a marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
|
|
|
GTX133724 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SMARCA2 antibody (GTX133724) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
|
|
|
GTX133724 ICC/IF Image
SMARCA2 antibody detects SMARCA2 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SMARCA2 stained by SMARCA2 antibody (GTX133724) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Scale bar= 10 μm.
|
|
|
GTX133724 IHC-P Image
SMARCA2 antibody detects SMARCA2 protein at nucleus in mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
SMARCA2 antibody (GTX133724) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
|
GTX133724 IHC-P Image
SMARCA2 antibody detects SMARCA2 protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
SMARCA2 antibody (GTX133724) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
REFERENCE
There are currently no references for SMARCA2 antibody (GTX133724). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for SMARCA2 antibody (GTX133724). Be the first to share your experience with this product.
