GeneTex
United States (US)

SMARCC1 antibody [C2C3], C-term

KO/KD
GTX114777_41332_20180316_WB_KO_watermark_18121410_434.jpg
GTX114777_40667_20160421_WB_M_18121410_446.jpg
GTX114777_40667_IFA_18121410_224.jpg
GTX114777_40667_IHC_18121410_201.jpg
GTX114777_41332_IHC_R_18121410_780.jpg
GTX114777_41332_20151223_IFA_18121410_221.jpg
GTX114777_41332_IHC_M_2_18121410_874.jpg
GTX114777_41332_IHC_M_18121410_257.jpg
GTX114777_40667_IHC_R_18121410_240.jpg
GTX114777_40667_IP_18121410_327.jpg
GTX114777_41332_WB_18121410_699.jpg
GTX114777_40667_ChIP_18121410_639.jpg
GTX114777_40667_IHC_M_18121410_290.jpg

Cat No. GTX114777

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP, ChIP assay
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

123 kDa. ( Note )

Positive Control

HeLa , HepG2 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human SMARCC1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Swi/Snf Related, Matrix Associated, Actin Dependent Regulator Of Chromatin Subfamily C Member 1,Baf155,Cracc1,Rsc8,Srg3,Swi3,Smarcc1

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and contains a predicted leucine zipper motif typical of many transcription factors. [provided by RefSeq]

Database

DATA IMAGES
GTX114777_41332_20180316_WB_KO_watermark_18121410_434.jpg

GTX114777 WB Image

KO/KD

Wild-type (WT) and SMARCC1 knockout (KO) HeLa cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with SMARCC1 antibody [C2C3], C-term (GTX114777) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CRISPR knockout cell lines generated by www.edigene.com

GTX114777_40667_20160421_WB_M_18121410_446.jpg

GTX114777 WB Image

Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with SMARCC1 antibody [C2C3], C-term (GTX114777) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX114777_40667_IFA_18121410_224.jpg

GTX114777 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using SMARCC1(GTX114777) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

GTX114777_40667_IHC_18121410_201.jpg

GTX114777 IHC-P Image

Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using SMARCC1(GTX114777) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX114777_41332_IHC_R_18121410_780.jpg

GTX114777 IHC-P Image

SMARCC1 antibody [C2C3], C-term detects SMARCC1 protein at nucleus on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
SMARCC1 antibody [C2C3], C-term (GTX114777) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX114777_41332_20151223_IFA_18121410_221.jpg

GTX114777 ICC/IF Image

SMARCC1 antibody [C2C3], C-term detects SMARCC1 protein at nucleus by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SMARCC1 protein stained by SMARCC1 antibody [C2C3], C-term (GTX114777) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.

GTX114777_41332_IHC_M_2_18121410_874.jpg

GTX114777 IHC-P Image

SMARCC1 antibody [C2C3], C-term detects SMARCC1 protein at nucleus on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
SMARCC1 antibody [C2C3], C-term (GTX114777) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX114777_41332_IHC_M_18121410_257.jpg

GTX114777 IHC-P Image

SMARCC1 antibody [C2C3], C-term detects SMARCC1 protein at nucleus on mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
SMARCC1 antibody [C2C3], C-term (GTX114777) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX114777_40667_IHC_R_18121410_240.jpg

GTX114777 IHC-P Image

Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using SMARCC1(GTX114777) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX114777_40667_IP_18121410_327.jpg

GTX114777 IP Image

SMARCC1 antibody immunoprecipitates SMARCC1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 30 μg whole cell lysate/extract of SMARCC1 protein expressing 293T cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of SMARCC1 by 2.5 μg of SMARCC1 antibody (GTX114777) 5% SDS-PAGE The immunoprecipitated SMARCC1 protein was detected by SMARCC1 antibody (GTX114777) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

GTX114777_41332_WB_18121410_699.jpg

GTX114777 WB Image

SMARCC1 antibody detects SMARCC1 protein by Western blot analysis.
A. 30 μg HeLa whole cell lysate/extract
B. 30 μg HepG2 whole cell lysate/extract
5 % SDS-PAGE
SMARCC1 antibody (GTX114777) dilution: 1:10000

GTX114777_40667_ChIP_18121410_639.jpg

GTX114777 ChIP assay Image

Cross-linked ChIP was performed with MF-7 chromatin extract treated with B-estradiol (10 nM for 45 min) and 5 μg of either control rabbit IgG or anti-SMARCC1 antibody. The precipitated DNA was detected by PCR with primer set targeting to ESR1 or Sat2.

GTX114777_40667_IHC_M_18121410_290.jpg

GTX114777 IHC-P Image

Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using SMARCC1(GTX114777) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169