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SMARCC1 antibody [C2C3], C-term

KO/KD
Anti-SMARCC1 antibody [C2C3], C-term used in Western Blot (WB). GTX114777
Anti-SMARCC1 antibody [C2C3], C-term used in Western Blot (WB). GTX114777
Anti-SMARCC1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX114777
Anti-SMARCC1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX114777
Anti-SMARCC1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX114777
Anti-SMARCC1 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114777
Anti-SMARCC1 antibody [C2C3], C-term used in Western Blot (WB). GTX114777
Anti-SMARCC1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX114777
Anti-SMARCC1 antibody [C2C3], C-term used in Immunoprecipitation (IP). GTX114777
Anti-SMARCC1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX114777
Anti-SMARCC1 antibody [C2C3], C-term used in ChIP assay (ChIP assay). GTX114777
Anti-SMARCC1 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114777
Anti-SMARCC1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX114777

Cat. No. GTX114777

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, ChIP assay

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all SMARCC1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

123 kDa. ( Note )

Positive Control

Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.77 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human SMARCC1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11164873

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SWI/SNF related, matrix associated, actin dependent regulator of chromatin subfamily c member 1 , BAF155 , CRACC1 , Rsc8 , SRG3 , SWI3

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and contains a predicted leucine zipper motif typical of many transcription factors. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SMARCC1 antibody [C2C3], C-term used in Western Blot (WB). GTX114777

GTX114777 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SMARCC1 antibody [C2C3], C-term (GTX114777) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SMARCC1 antibody [C2C3], C-term used in Western Blot (WB). GTX114777

GTX114777 WB Image

KO/KD

Wild-type (WT) and SMARCC1 knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SMARCC1 antibody [C2C3], C-term (GTX114777) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SMARCC1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX114777

GTX114777 IHC-P Image

Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using SMARCC1(GTX114777) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SMARCC1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX114777

GTX114777 IHC-P Image

SMARCC1 antibody [C2C3], C-term detects SMARCC1 protein at nucleus on mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
SMARCC1 antibody [C2C3], C-term (GTX114777) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SMARCC1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX114777

GTX114777 IHC-P Image

Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using SMARCC1(GTX114777) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SMARCC1 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114777

GTX114777 ICC/IF Image

SMARCC1 antibody [C2C3], C-term detects SMARCC1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SMARCC1 stained by SMARCC1 antibody [C2C3], C-term (GTX114777) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar= 10 μm.

Anti-SMARCC1 antibody [C2C3], C-term used in Western Blot (WB). GTX114777

GTX114777 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SMARCC1 antibody [C2C3], C-term (GTX114777) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SMARCC1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX114777

GTX114777 IHC-P Image

SMARCC1 antibody [C2C3], C-term detects SMARCC1 protein at nucleus on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
SMARCC1 antibody [C2C3], C-term (GTX114777) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SMARCC1 antibody [C2C3], C-term used in Immunoprecipitation (IP). GTX114777

GTX114777 IP Image

SMARCC1 antibody immunoprecipitates SMARCC1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 30 μg whole cell lysate/extract of SMARCC1 protein expressing 293T cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of SMARCC1 by 2.5 μg of SMARCC1 antibody (GTX114777) 5% SDS-PAGE The immunoprecipitated SMARCC1 protein was detected by SMARCC1 antibody (GTX114777) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Anti-SMARCC1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX114777

GTX114777 IHC-P Image

SMARCC1 antibody [C2C3], C-term detects SMARCC1 protein at nucleus on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
SMARCC1 antibody [C2C3], C-term (GTX114777) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SMARCC1 antibody [C2C3], C-term used in ChIP assay (ChIP assay). GTX114777

GTX114777 ChIP assay Image

Cross-linked ChIP was performed with MF-7 chromatin extract treated with B-estradiol (10 nM for 45 min) and 5 μg of either control rabbit IgG or anti-SMARCC1 antibody. The precipitated DNA was detected by PCR with primer set targeting to ESR1 or Sat2.

Anti-SMARCC1 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114777

GTX114777 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using SMARCC1(GTX114777) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-SMARCC1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX114777

GTX114777 IHC-P Image

Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using SMARCC1(GTX114777) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Cai X et al. Cancer Cell Int 2021; 21 (1) : 701 Prognostic biomarker SMARCC1 and its association with immune infiltrates in hepatocellular carcinoma.
Application : IHC-P
Reactivity : Human
Li HJ et al. Oncogene 2016; 35 (17) : 2266-78 NKX6.1 functions as a metastatic suppressor through epigenetic regulation of the epithelial-mesenchymal transition.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169