GeneTex
United States (US)

SMARCC2 / BAF170 antibody

GTX114778_42179_20151221_IHC-P_M_18121410_463.jpg
GTX114778_42319_20151210_WB_18121410_214.jpg
GTX114778_42179_20151221_IHC-P_R_18121410_724.jpg

Cat No. GTX114778

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

133 kDa. ( Note )

Positive Control

MCF-7 , MDA-MB-231

Predict Reactivity

Bovine, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.37 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human SMARCC2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Swi/Snf Related, Matrix Associated, Actin Dependent Regulator Of Chromatin Subfamily C Member 2,Baf170,Cracc2,Rsc8,Smarcc2

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and contains a predicted leucine zipper motif typical of many transcription factors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

DATA IMAGES
GTX114778_42179_20151221_IHC-P_M_18121410_463.jpg

GTX114778 IHC-P Image

SMARCC2 antibody detects SMARCC2 protein at nucleolus in mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
SMARCC2 antibody (GTX114778) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX114778_42319_20151210_WB_18121410_214.jpg

GTX114778 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SMARCC2 antibody (GTX114778) diluted at 1:1000.

GTX114778_42179_20151221_IHC-P_R_18121410_724.jpg

GTX114778 IHC-P Image

SMARCC2 antibody detects SMARCC2 protein at nucleolus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
SMARCC2 antibody (GTX114778) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REVIEW

SMARCC2 / BAF170 antibody Cat No. GTX114778

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 05-Nov-2012
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa cells
Amount used : 10μg
Blocking : Thermo protein-free blocking buffer , 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 18Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169