GeneTex
United States (US)

SMARCD1 antibody [N2C1], Internal

Orthogonal
GTX114780_IFA_18121410_537.jpg
GTX114780_40233_20161013_WB_Fraction_18121410_862.jpg
GTX114780_40233_WB_M_18121410_125.jpg

Cat No. GTX114780

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Positive Control

HeLa , HeLa nuclear extract , NIH-3T3

Predict Reactivity

Rat, Zebrafish, Bovine, Pig, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.84 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SMARCD1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Swi/Snf Related, Matrix Associated, Actin Dependent Regulator Of Chromatin, Subfamily D, Member 1,Baf60A,Cracd1,Rsc6P,Smarcd1

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and has sequence similarity to the yeast Swp73 protein. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

DATA IMAGES
GTX114780_IFA_18121410_537.jpg

GTX114780 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using SMARCD1(GTX114780) antibody at 1:200 dilution.

GTX114780_40233_20161013_WB_Fraction_18121410_862.jpg

GTX114780 WB Image

Orthogonal

HeLa whole cell and nuclear extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with SMARCD1 antibody [N2C1], Internal (GTX114780) diluted at 1:1000.

GTX114780_40233_WB_M_18121410_125.jpg

GTX114780 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
GTX114780 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169