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SMC1 antibody [5B6]

Anti-SMC1 antibody [5B6] used in Western Blot (WB). GTX82813
Anti-SMC1 antibody [5B6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82813
Anti-SMC1 antibody [5B6] used in IHC (Paraffin sections) (IHC-P). GTX82813
Anti-SMC1 antibody [5B6] used in Flow cytometry (FACS). GTX82813

Cat. No. GTX82813

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5B6

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all SMC1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

143 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human SMC1 expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

RRID

AB_11171708

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

structural maintenance of chromosomes 1A , CDLS2 , DXS423E , SB1.8 , SMC1 , SMC1L1 , SMC1alpha , SMCB

Cellular Localization

Nucleus

Background

Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1L2 or the protein encoded by this gene. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Therefore, the encoded protein is thought to be an important part of functional kinetochores. In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair. This gene, which belongs to the SMC gene family, is located in an area of the X-chromosome that escapes X inactivation. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-SMC1 antibody [5B6] used in Western Blot (WB). GTX82813

GTX82813 WB Image

WB analysis of K562 (1), Jurkat (2) and A549 (3) cell lysate using GTX82813 SMC1 antibody [5B6].

Anti-SMC1 antibody [5B6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82813

GTX82813 ICC/IF Image

ICC/IF analysis of NIH3T3 cells using GTX82813 SMC1 antibody [5B6].
Green : SMC1
Red: Actin filaments

Anti-SMC1 antibody [5B6] used in IHC (Paraffin sections) (IHC-P). GTX82813

GTX82813 IHC-P Image

IHC-P analysis of human colon using GTX82813 SMC1 antibody [5B6].

Anti-SMC1 antibody [5B6] used in Flow cytometry (FACS). GTX82813

GTX82813 FACS Image

FACS analysis of HeLa cells using GTX82813 SMC1 antibody [5B6].
Green : SMC1
Purple : negative control

REFERENCE

Application Reference

Sarkar A et al. Mol Cell Biochem 2020; Activation of ATM kinase by ROS generated during ionophore-induced mitophagy in human T and B cell malignancies.
Application : WB
Sarkar A et al. Haematologica 2020; (Epub) Ataxia telangiectasia mutated interacts with Parkin and induces mitophagy independent of kinase activity. Evidence from mantle cell lymphoma.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399