GeneTex
  • Country / Location Selection

United States (US)

SMC1 (phospho Ser957) antibody

Anti-SMC1 (phospho Ser957) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55474
Anti-SMC1 (phospho Ser957) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55474
Anti-SMC1 (phospho Ser957) antibody used in Western Blot (WB). GTX55474
Anti-SMC1 (phospho Ser957) antibody used in Western Blot (WB). GTX55474
Anti-SMC1 (phospho Ser957) antibody used in IHC (Paraffin sections) (IHC-P). GTX55474
Anti-SMC1 (phospho Ser957) antibody used in Immunoprecipitation (IP). GTX55474

Cat No. GTX55474

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:20 - 1:100
IHC-P 1:50 - 1:200
IP 1:50 - 1:100
Not tested in other applications.

Calculated MW

143 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A phospho specific peptide corresponding to residues surrounding S957 of human SMC1A

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

structural maintenance of chromosomes 1A , CDLS2 , DXS423E , SB1.8 , SMC1 , SMC1L1 , SMC1alpha , SMCB

Cellular Localization

Nucleus

Background

Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1B or the protein encoded by this gene. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Therefore, the encoded protein is thought to be an important part of functional kinetochores. In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair. This gene, which belongs to the SMC gene family, is located in an area of the X-chromosome that escapes X inactivation. Mutations in this gene result in Cornelia de Lange syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]

Database

Research Area

DATA IMAGES

Anti-SMC1 (phospho Ser957) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55474

GTX55474 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX55474 SMC1 (phospho Ser957) antibody.
Blue : DAPI

Anti-SMC1 (phospho Ser957) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55474

GTX55474 ICC/IF Image

ICC/IF analysis of GFP-RNF168 transgenic U2OS cells using GTX55474 SMC1 (phospho Ser957) antibody.
Green : GFP-RNF168 fusion protein expression for DNA damage marker
Blue : DAPI

Anti-SMC1 (phospho Ser957) antibody used in Western Blot (WB). GTX55474

GTX55474 WB Image

WB analysis of HeLa and NIH/3T3 cells treated by UV for 15-30 minutes using GTX55474 SMC1 (phospho Ser957) antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-SMC1 (phospho Ser957) antibody used in Western Blot (WB). GTX55474

GTX55474 WB Image

WB analysis of HeLa cells treated by UV at room temperature for 15-30 minutes using GTX55474 SMC1 (phospho Ser957) antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-SMC1 (phospho Ser957) antibody used in IHC (Paraffin sections) (IHC-P). GTX55474

GTX55474 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX55474 SMC1 (phospho Ser957) antibody.
Dilution : 1:100

Anti-SMC1 (phospho Ser957) antibody used in Immunoprecipitation (IP). GTX55474

GTX55474 IP Image

IP analysis of HeLa cell lysate using GTX55474 SMC1 (phospho Ser957) antibody.
Antibody amount : 3μg / 200μg lysate
Dilution : 1:1000

REFERENCE

There are currently no references for SMC1 (phospho Ser957) antibody (GTX55474). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for SMC1 (phospho Ser957) antibody (GTX55474). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339