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SMC1 (phospho Ser966) antibody

Orthogonal
Anti-SMC1 (phospho Ser966) antibody used in Western Blot (WB). GTX21276
Anti-SMC1 (phospho Ser966) antibody used in Proximity ligation assay (PLA). GTX21276
Anti-SMC1 (phospho Ser966) antibody used in IHC (Paraffin sections) (IHC-P). GTX21276
Anti-SMC1 (phospho Ser966) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX21276
Anti-SMC1 (phospho Ser966) antibody used in Western Blot (WB). GTX21276

Cat. No. GTX21276

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, ELISA, PLA

Reactivity

 Human, Mouse
Package
50 μg ($399)
See all SMC1A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:5000
ICC/IF 1:250 - 1:1000
IHC-P 1:500 - 1:2000
IP 2 - 6 μg/mg lysate
ELISA 1:100 - 1:2000
PLA 1:1000 - 1:20000
Not tested in other applications.

Calculated MW

143 kDa. ( Note )

Predict Reactivity

Monkey, Gibbon, Macaque, Marmoset, Orangutan(>80% identity)

PROPERTIES

Form

Liquid

Buffer

Tris-Citrate/Phosphate

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Immunogen for this antibody was a phosphorylated synthetic peptide, which represented a portion of human Structural Maintenance of Chromosomes 1 (GeneID 8243) around serine 966 according to the numbering given in entry NP_006297.2.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_373026

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

structural maintenance of chromosomes 1A , CDLS2 , DXS423E , SB1.8 , SMC1 , SMC1L1 , SMC1alpha , SMCB

Cellular Localization

Nucleus

Background

Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1L2 or the protein encoded by this gene. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Therefore, the encoded protein is thought to be an important part of functional kinetochores. In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair. This gene, which belongs to the SMC gene family, is located in an area of the X-chromosome that escapes X inactivation. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SMC1 (phospho Ser966) antibody used in Western Blot (WB). GTX21276

GTX21276 WB Image

WB analysis of HeLa cells (30 μg/lane) treated with 10 Gy ionizing irradiation using GTX21276 SMC1 (phospho Ser966) antibody.

Anti-SMC1 (phospho Ser966) antibody used in Proximity ligation assay (PLA). GTX21276

GTX21276 PLA Image

PLA analysis of HeLa cells using GTX21276 SMC1 (phospho Ser966) antibody and anti-human SA1 antibody.
Red : positive signal
Blue : DAPI

Anti-SMC1 (phospho Ser966) antibody used in IHC (Paraffin sections) (IHC-P). GTX21276

GTX21276 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX21276 SMC1 (phospho Ser966) antibody.
Dilution : 1 μg/ml

Anti-SMC1 (phospho Ser966) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX21276

GTX21276 ICC/IF Image

ICC/IF analysis of Neocarzinostatin treated asynchronous HeLa cells using GTX21276 SMC1 (phospho Ser966) antibody.
Red : Primary antibody
Blue : DAPI

Anti-SMC1 (phospho Ser966) antibody used in Western Blot (WB). GTX21276

GTX21276 WB Image

Orthogonal

WB analysis of NCS treated(+; 200 ng/ml; 1h) or mock treated (-) HeLa cell using GTX21276 SMC1 (phospho Ser966) antibody.
Dilution : 0.1 μg/ml

REFERENCE

Application Reference

Sarkar A et al. Mol Cell Biochem 2020; Activation of ATM kinase by ROS generated during ionophore-induced mitophagy in human T and B cell malignancies.
Application : WB
Sarkar A et al. Haematologica 2020; (Epub) Ataxia telangiectasia mutated interacts with Parkin and induces mitophagy independent of kinase activity. Evidence from mantle cell lymphoma.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399