SMC1A antibody [8E6-A4-F3]

Cat No. GTX49176

Host	Mouse
Clonality	Monoclonal
Clone Name	8E6-A4-F3
Isotype	IgG1
Application	WB, IHC-P, FACS
Reactivity	Human
Package	100 μg ($339)

See all SMC1A products

Datasheet
Document

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	Assay dependent
IHC-P	Assay dependent
FACS	Assay dependent

Not tested in other applications.

Calculated MW

143 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7.4), 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant human SMC1A protein fragments expressed in E.coli.

Purification

Affinity purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

structural maintenance of chromosomes 1A , CDLS2 , DXS423E , SB1.8 , SMC1 , SMC1L1 , SMC1alpha , SMCB

Cellular Localization

Nucleus

Background

Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1B or the protein encoded by this gene. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Therefore, the encoded protein is thought to be an important part of functional kinetochores. In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair. This gene, which belongs to the SMC gene family, is located in an area of the X-chromosome that escapes X inactivation. Mutations in this gene result in Cornelia de Lange syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]

Database

Gene ID: 8243 SMC1A
UniProt: Q14683 SMC1A

Research Area

DATA IMAGES

GTX49176 IHC-P Image

IHC-P analysis of human colon tissue using SMC1A antibody.

GTX49176 FACS Image

FACS analysis of HeLa cells using SMC1A antibody at a dilution of 1:100 (red) or an isotype control (black).

GTX49176 WB Image

WB analysis of HeLaNE, Jurkat and A431 lysates using SMC1A antibody at a dilution of 1:1,000.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μg

$ 339

Qty