*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji
Mouse, Rat, Zebrafish, Bovine, Chicken, Xenopus laevis(>80% identity)
0.1M Tris-Glycine (pH7), 10% Glycerol
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the N-terminus region of human SMC1A. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
structural maintenance of chromosomes 1A , CDLS2 , DXS423E , SB1.8 , SMC1 , SMC1L1 , SMC1alpha , SMCB
Nucleus , Kinetochore
Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1L2 or the protein encoded by this gene. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Therefore, the encoded protein is thought to be an important part of functional kinetochores. In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair. This gene, which belongs to the SMC gene family, is located in an area of the X-chromosome that escapes X inactivation. [provided by RefSeq]
GTX109724 IHC-P Image
Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using SMC1A(GTX109724) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX109724 IP Image
Immunoprecipitation of SMC1A protein from 293T whole cell extracts using 5 μg of SMC1A antibody [N1N2], N-term (GTX109724).
Western blot analysis was performed using SMC1A antibody [N1N2], N-term (GTX109724) diluted at 1:1000.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
GTX109724 WB Image
Sample (30 ug of whole cell lysate)
A: Molt-4 (GTX27912)
7.5% SDS PAGE
GTX109724 diluted at 1:5000